{"id":"W4290767943","doi":"10.1016/j.ab.2022.114841","title":"Filter paper disks as a matrix for manipulation of recombinant proteins","year":2022,"lang":"en","type":"article","venue":"Analytical Biochemistry","topic":"Transgenic Plants and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":false,"ca_institutions":"Western University","funders":"Western University","keywords":"Linker; Elution; Fusion protein; Clostridium thermocellum; Chromatography; Chemistry; Recombinant DNA; Covalent bond; Binding domain; Matrix (chemical analysis); Protease; Biochemistry; Cellulose; Binding site; Enzyme; Gene; Computer science; Organic chemistry; Cellulase","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00009430249,0.00009389956,0.0001106528,0.00001398207,0.00009759476,0.000006991963,0.0001651724,0.00007127847,0.0002425045],"category_scores_gemma":[0.00003460483,0.00009068577,0.0001334189,0.00007187344,0.00004345508,0.000001771783,0.00007313839,0.00007040773,0.000003270675],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001365826,"about_ca_system_score_gemma":0.00006152516,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001482717,"about_ca_topic_score_gemma":0.000001790726,"domain_scores_codex":[0.999249,0.0000116592,0.0002027923,0.0002683367,0.0001139051,0.0001542462],"domain_scores_gemma":[0.9995525,0.0000132944,0.00006185212,0.0002785381,0.00003694089,0.00005684794],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001210722,0.0001666347,0.0002603212,0.00004079263,0.00005339965,7.646059e-7,0.000007293049,0.00004085215,0.9931638,0.001165865,0.00448252,0.0004966508],"study_design_scores_gemma":[0.0007749038,0.0002986007,0.000552532,0.000008093268,0.00007809512,0.00002726721,0.00008687204,0.0006448113,0.8971643,0.001056851,0.09906377,0.0002439348],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9943744,0.0001730041,0.00253889,0.0008324226,0.00005061107,0.000383008,0.0002849691,0.00001156759,0.001351142],"genre_scores_gemma":[0.9938242,0.00001498574,0.0005341452,0.0001255165,0.00006438401,0.0002072855,0.0007999974,0.00001279725,0.004416676],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.09599956,"threshold_uncertainty_score":0.3698058,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01502426555680787,"score_gpt":0.2889285257733919,"score_spread":0.273904260216584,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}