{"id":"W4291021353","doi":"10.1098/rsob.220149","title":"A genetic model for <i>in vivo</i> proximity labelling of the mammalian secretome","year":2022,"lang":"en","type":"article","venue":"Open Biology","topic":"Biotin and Related Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"National Institute of Diabetes and Digestive and Kidney Diseases; National Institute of General Medical Sciences; Howard Hughes Medical Institute","keywords":"Biology; Endoplasmic reticulum; Biotinylation; Proteome; Secretory pathway; Secretory protein; Cell biology; Golgi apparatus; Ectodomain; Signal peptide; Secretion; Signal transduction; Proteomics; Biochemistry; Peptide sequence; Receptor; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005352907,0.0008262045,0.0003347257,0.0008425114,0.0005131557,0.0008273295,0.000970062,0.001065315,0.002999164],"category_scores_gemma":[0.0003021916,0.000436572,0.0006220324,0.0003983454,0.000995487,0.0006152432,0.0007357208,0.001909934,0.00168684],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007258789,"about_ca_system_score_gemma":0.000528782,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001118472,"about_ca_topic_score_gemma":0.001698054,"domain_scores_codex":[0.9994405,0.00008772089,0.00006581072,0.0001806807,0.0001468758,0.00007841286],"domain_scores_gemma":[0.9993379,0.00009028521,0.0002428711,0.0001275729,0.00006470047,0.0001366274],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005816908,0.00002066034,0.00007299246,0.00002358817,0.000004420106,0.00005290257,0.00001740932,0.00004629791,0.9980876,0.0008990329,0.00006953839,0.000647412],"study_design_scores_gemma":[0.00002354924,0.0001894793,0.001192844,0.00001635274,0.00002433959,0.0006129874,0.0000358472,0.001348922,0.9842798,0.0002812776,0.01198128,0.00001340029],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.770628,0.002022395,0.2063218,0.001107802,0.0006533461,0.0006219835,0.005078292,0.002344596,0.01122172],"genre_scores_gemma":[0.8616531,0.001993698,0.1032607,0.0004835296,0.00006297095,0.0007378435,0.004344416,0.001009726,0.02645405],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002999164,"threshold_uncertainty_score":0.01003319,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02073986041691228,"score_gpt":0.2734393944962495,"score_spread":0.2526995340793373,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}