{"id":"W4291021353","doi":"10.1098/rsob.220149","title":"A genetic model for <i>in vivo</i> proximity labelling of the mammalian secretome","year":2022,"lang":"en","type":"article","venue":"Open Biology","topic":"Biotin and Related Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"National Institute of Diabetes and Digestive and Kidney Diseases; National Institute of General Medical Sciences; Howard Hughes Medical Institute","keywords":"Biology; Endoplasmic reticulum; Biotinylation; Proteome; Secretory pathway; Secretory protein; Cell biology; Golgi apparatus; Ectodomain; Signal peptide; Secretion; Signal transduction; Proteomics; Biochemistry; Peptide sequence; Receptor; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002140022,0.00008396572,0.0001436457,0.00001661158,0.0001622351,0.00000474208,0.0005088864,0.0001011723,0.00002312705],"category_scores_gemma":[0.00002015491,0.00006001483,0.0000666758,0.00008514395,0.00009472253,0.000001078137,0.0008536276,0.000102718,3.32816e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001083027,"about_ca_system_score_gemma":0.00006685525,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005727594,"about_ca_topic_score_gemma":0.0001287828,"domain_scores_codex":[0.9992715,0.00009344439,0.0001723378,0.0002454162,0.00003417215,0.0001831242],"domain_scores_gemma":[0.9996288,0.0000100394,0.00008471686,0.0002327558,0.00002678346,0.00001683979],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002383454,0.000131859,0.01375941,0.00003003105,0.00007974532,6.619118e-7,0.0002830903,0.003387419,0.9773455,0.001335392,0.002727466,0.0006810893],"study_design_scores_gemma":[0.006686814,0.002316894,0.004262094,0.00004124251,0.00012526,0.00006368196,0.001274984,0.03769089,0.6535276,0.01124381,0.2816723,0.001094384],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9931211,0.001256911,0.001008856,0.0008009679,0.0002463655,0.0010014,0.0002377848,0.000003404787,0.002323245],"genre_scores_gemma":[0.9950019,0.000111198,0.001678121,0.0004046344,0.00002679879,0.0001631094,0.00001801253,0.00001074301,0.00258547],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.3238178,"threshold_uncertainty_score":0.2447334,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02073986041691228,"score_gpt":0.2734393944962495,"score_spread":0.2526995340793373,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}