{"id":"W4291915012","doi":"10.1101/2022.08.15.501889","title":"Development and applications of a CRISPR activation system for facile genetic overexpression in <i>Candida albicans</i>","year":2022,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Antifungal resistance and susceptibility","field":"Medicine","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"Natural Sciences and Engineering Research Council of Canada; Mitacs; University of Guelph; University of Notre Dame; University of Minnesota; Canadian Institute for Advanced Research; Saint Louis University; Burroughs Wellcome Fund","keywords":"Candida albicans; Biology; CRISPR; Gene; Antifungal drug; Corpus albicans; RNA; Virulence; Computational biology; Cas9; Phenotype; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003838183,0.000413429,0.0002550665,0.0003184968,0.0003666324,0.0006016793,0.0004116687,0.0004172038,0.001682966],"category_scores_gemma":[0.0002684972,0.000268751,0.0003539757,0.000179801,0.0002403258,0.0001771203,0.0004219898,0.0008533193,0.001076509],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004349814,"about_ca_system_score_gemma":0.0004943831,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001228241,"about_ca_topic_score_gemma":0.00119849,"domain_scores_codex":[0.9997001,0.00004044699,0.00003699465,0.00007679283,0.0001119911,0.00003377021],"domain_scores_gemma":[0.9998677,0.0000298596,0.00002447175,0.00003039966,0.00002238627,0.00002525598],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002314288,0.00001346489,0.00009065516,0.00002943255,0.000003428185,0.00004754485,0.00001236064,0.0002281911,0.9957179,0.0002617257,0.0002553843,0.003316711],"study_design_scores_gemma":[0.000006393789,0.00004528951,0.0005299915,0.000005739986,0.000005987398,0.0001805273,0.000007949599,0.001661772,0.9918742,0.00005798397,0.005616319,0.000007872013],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6825245,0.002032315,0.2851103,0.001407564,0.0004105548,0.0007006194,0.004508501,0.005981775,0.01732392],"genre_scores_gemma":[0.7840673,0.001204874,0.1988626,0.0002695664,0.00004280861,0.0003367102,0.003137899,0.000795064,0.01128323],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001682966,"threshold_uncertainty_score":0.005630076,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01492451095676489,"score_gpt":0.2471661777703625,"score_spread":0.2322416668135976,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}