{"id":"W4293105417","doi":"10.1007/s12035-022-03003-1","title":"Antagonism of the Muscarinic Acetylcholine Type 1 Receptor Enhances Mitochondrial Membrane Potential and Expression of Respiratory Chain Components via AMPK in Human Neuroblastoma SH-SY5Y Cells and Primary Neurons","year":2022,"lang":"en","type":"article","venue":"Molecular Neurobiology","topic":"Ion channel regulation and function","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba; St. Boniface Hospital","funders":"Institute of Neurosciences, Mental Health and Addiction; Canadian Institutes of Health Research; Mitacs","keywords":"AMPK; Muscarinic acetylcholine receptor; Endocrinology; Pirenzepine; Internal medicine; Cell biology; Biology; Mitochondrion; SH-SY5Y; Acetylcholine; Chemistry; Protein kinase A; Receptor; Medicine; Cell culture; Phosphorylation; Neuroblastoma","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002123175,0.0003176087,0.0004696614,0.0001953457,0.0002233555,0.0002482242,0.0001934371,0.0002440347,0.001340442],"category_scores_gemma":[0.00008143168,0.0001519343,0.0003847633,0.0002013053,0.0001739494,0.0001849035,0.0001592212,0.0005806147,0.0004623311],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004763789,"about_ca_system_score_gemma":0.0003606772,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002898473,"about_ca_topic_score_gemma":0.00599822,"domain_scores_codex":[0.9998037,0.00001699811,0.00003653251,0.00003932718,0.00005796039,0.00004550822],"domain_scores_gemma":[0.999946,0.000008806201,0.00001333281,0.000006894337,0.00001049194,0.00001443105],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001517687,0.00004178029,0.0001214708,0.00005599908,0.000006909245,0.00004067932,0.00002121803,0.00004096234,0.9989669,0.00005325983,0.00006269715,0.000436316],"study_design_scores_gemma":[0.00005715355,0.0007076241,0.006920562,0.0000128558,0.00003920636,0.0001725114,0.00007123825,0.001084964,0.9886737,0.0000308711,0.00222394,0.000005286407],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.992861,0.002546995,0.001242226,0.0001253378,0.00007385172,0.000042326,0.001399812,0.0000477886,0.00166073],"genre_scores_gemma":[0.9884387,0.002830695,0.002593396,0.00007334013,0.00001897983,0.000134684,0.001876468,0.00001305888,0.004020625],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002898473,"threshold_uncertainty_score":0.005763173,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008206717712317391,"score_gpt":0.2170265656635751,"score_spread":0.2088198479512577,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}