{"id":"W4293105417","doi":"10.1007/s12035-022-03003-1","title":"Antagonism of the Muscarinic Acetylcholine Type 1 Receptor Enhances Mitochondrial Membrane Potential and Expression of Respiratory Chain Components via AMPK in Human Neuroblastoma SH-SY5Y Cells and Primary Neurons","year":2022,"lang":"en","type":"article","venue":"Molecular Neurobiology","topic":"Ion channel regulation and function","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba; St. Boniface Hospital","funders":"Institute of Neurosciences, Mental Health and Addiction; Canadian Institutes of Health Research; Mitacs","keywords":"AMPK; Muscarinic acetylcholine receptor; Endocrinology; Pirenzepine; Internal medicine; Cell biology; Biology; Mitochondrion; SH-SY5Y; Acetylcholine; Chemistry; Protein kinase A; Receptor; Medicine; Cell culture; Phosphorylation; Neuroblastoma","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001241449,0.000150765,0.0002189216,0.00009803876,0.0001152906,0.000004887971,0.0001796325,0.0001125341,0.00002477451],"category_scores_gemma":[0.0000281281,0.0001360689,0.00006865649,0.0001372631,0.0002168767,0.000005225609,0.0005028872,0.0001905718,2.610997e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00000830011,"about_ca_system_score_gemma":0.00002937902,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001327544,"about_ca_topic_score_gemma":0.000003868144,"domain_scores_codex":[0.9985189,0.0004774004,0.0003263509,0.0003969504,0.0001157498,0.0001646724],"domain_scores_gemma":[0.9994121,0.000017027,0.0002134521,0.0002795607,0.00004366987,0.00003418186],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003829791,0.00009162694,0.0001548365,0.00002505333,0.000007760008,0.0000027421,0.00003097309,0.0009080127,0.9981756,0.00001119593,0.0001191121,0.00009013352],"study_design_scores_gemma":[0.001057139,0.0006845537,0.003477433,0.000006741656,0.0000113949,0.00001878803,0.00001510011,0.00009439863,0.9910805,0.000008711315,0.003436641,0.0001085525],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9980423,0.0001233443,0.0001380152,0.00009584929,0.001234767,0.0002957427,0.00003274059,0.000006333054,0.00003083339],"genre_scores_gemma":[0.9990971,0.00003884737,0.00003871008,0.0005098919,0.00008990557,0.00001477016,0.0001068637,0.00002075159,0.00008313548],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.007095028,"threshold_uncertainty_score":0.554873,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008206717712317391,"score_gpt":0.2170265656635751,"score_spread":0.2088198479512577,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}