{"id":"W4293384775","doi":"10.1016/j.xpro.2022.101638","title":"Protocol to derive human trophoblast stem cells directly from primed pluripotent stem cells","year":2022,"lang":"en","type":"article","venue":"STAR Protocols","topic":"Renal and related cancers","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; Sinai Health System; Lunenfeld-Tanenbaum Research Institute","funders":"Youth Innovation Promotion Association of the Chinese Academy of Sciences; Natural Science Foundation of Guangdong Province; National Natural Science Foundation of China","keywords":"Induced pluripotent stem cell; Reprogramming; Trophoblast; Stem cell; Biology; Human placenta; Cell biology; Computational biology; Embryonic stem cell; Placenta; Cell; Fetus; Genetics; Pregnancy; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007377407,0.0008629741,0.0006107349,0.001247013,0.001128918,0.0004646035,0.0009230144,0.0005398255,0.01928159],"category_scores_gemma":[0.0006097174,0.0007666902,0.0006326187,0.0007933555,0.0003824961,0.0003629664,0.0008497119,0.002248877,0.01428964],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003271355,"about_ca_system_score_gemma":0.00117989,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007652358,"about_ca_topic_score_gemma":0.002211818,"domain_scores_codex":[0.9993795,0.00009707428,0.0001081367,0.0001210756,0.0002355483,0.00005863003],"domain_scores_gemma":[0.999649,0.00007170009,0.00002396566,0.00009588962,0.00009561986,0.00006380532],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000598733,0.0002981448,0.0007857357,0.001159632,0.00005954655,0.001147829,0.0004042189,0.001058506,0.9121042,0.008976396,0.03401351,0.03939335],"study_design_scores_gemma":[0.0002155671,0.0007091581,0.004013871,0.0002260169,0.0001535915,0.002526845,0.00009035818,0.002291064,0.4191967,0.002576063,0.5678844,0.0001162858],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"protocol","genre_scores_codex":[0.09155215,0.0100235,0.7075059,0.001683905,0.002546661,0.02735024,0.06189847,0.007347781,0.09009136],"genre_scores_gemma":[0.1347815,0.01383496,0.5641659,0.001742128,0.0003271766,0.05992312,0.1326983,0.001564527,0.09096242],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.01928159,"threshold_uncertainty_score":0.06450337,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0154720358052391,"score_gpt":0.2728962727744999,"score_spread":0.2574242369692608,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}