{"id":"W4294344004","doi":"10.1101/2022.08.30.505962","title":"Knockout of <i>ykcB</i> , a putative glycosyltransferase, leads to vancomycin resistance in <i>Bacillus subtilis</i>","year":2022,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Bacterial biofilms and quorum sensing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Japan Society for the Promotion of Science; Institute of Genetics; Ryobi Teien Memory Foundation; Ichiro Kanehara Foundation for the Promotion of Medical Sciences and Medical Care; Takeda Science Foundation","keywords":"Bacillus subtilis; Microbiology; Vancomycin; Bacteria; Biology; Mutant; Gene knockout; Enterococcus faecium; Biofilm; Operon; Antibiotics; Staphylococcus aureus; Gene; Biochemistry; Genetics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001167159,0.0007468463,0.0003158555,0.00036272,0.0001652435,0.0004250839,0.0003393851,0.000380948,0.001217233],"category_scores_gemma":[0.0001658744,0.0002380223,0.0004630503,0.0002130797,0.0003142498,0.0001524614,0.0004313058,0.0007407647,0.0008139035],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003882839,"about_ca_system_score_gemma":0.0002724048,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002532721,"about_ca_topic_score_gemma":0.001592947,"domain_scores_codex":[0.9997318,0.00004307171,0.00003807095,0.0000419732,0.00009283717,0.00005219507],"domain_scores_gemma":[0.9997016,0.00004064996,0.0001241213,0.00002216512,0.00003007562,0.00008137395],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003952152,0.00001023947,0.00009484882,0.00001351503,0.000004173193,0.00003954897,0.000004402548,0.00002911557,0.9994892,0.00003074273,0.00001477247,0.0002299076],"study_design_scores_gemma":[0.00001783577,0.0001418176,0.004855876,0.00001473172,0.00002499282,0.000530529,0.00005060972,0.001394908,0.9910557,0.00003624658,0.001863015,0.00001364212],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9889442,0.0007242722,0.006874429,0.0001525604,0.00004502629,0.00004454825,0.001373579,0.000212813,0.001628561],"genre_scores_gemma":[0.9886844,0.0004147,0.00544541,0.00008833227,0.00001199593,0.00004225194,0.002281544,0.0001680599,0.002863461],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002532721,"threshold_uncertainty_score":0.005035937,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008818753287767633,"score_gpt":0.2186436931863097,"score_spread":0.2098249398985421,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}