{"id":"W4296690679","doi":"10.1038/s41589-022-01134-z","title":"Chemigenetic indicators based on synthetic chelators and green fluorescent protein","year":2022,"lang":"en","type":"article","venue":"Nature Chemical Biology","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Alberta","funders":"","keywords":"Fluorophore; Fluorescence; Chemistry; Chelation; Ligand (biochemistry); Target protein; Crown ether; Combinatorial chemistry; Biophysics; Ion; Biochemistry; Biology; Organic chemistry; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001210237,0.0009602582,0.0004440226,0.0005686688,0.0003758037,0.0008262607,0.001096809,0.001030457,0.001861388],"category_scores_gemma":[0.001358605,0.0004968808,0.0002664807,0.0003451704,0.0008712452,0.000770051,0.0008431363,0.001443924,0.00143252],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009769738,"about_ca_system_score_gemma":0.0006472964,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000236926,"about_ca_topic_score_gemma":0.000550445,"domain_scores_codex":[0.9992805,0.0001838657,0.00005391597,0.000145348,0.0002323738,0.0001038944],"domain_scores_gemma":[0.999136,0.000273679,0.0002218848,0.00008544815,0.0001634111,0.0001196076],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001396543,0.00001531137,0.00008342709,0.0001828665,0.000005840003,0.00004853421,0.00003595354,0.00007228728,0.9940575,0.002051906,0.0004903123,0.002816398],"study_design_scores_gemma":[0.00001182609,0.00003206901,0.00007042728,0.000004347864,0.000003814189,0.00005916494,0.00000551778,0.0002217889,0.996633,0.00009278279,0.002861696,0.000003492368],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.4361855,0.008892495,0.5240628,0.003144297,0.001106314,0.0007725709,0.00107673,0.004415555,0.02034381],"genre_scores_gemma":[0.714672,0.005474863,0.2540435,0.00134154,0.0002337779,0.0007943848,0.001854516,0.0006403092,0.02094499],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.001861388,"threshold_uncertainty_score":0.007088542,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003018789995491079,"score_gpt":0.2387290839870673,"score_spread":0.2357102939915762,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}