{"id":"W4297124146","doi":"10.1039/d2ay00840h","title":"Development of novel enzyme immobilization methods employing formaldehyde or triethoxysilylbutyraldehyde to fabricate immobilized enzyme microreactors for peptide mapping","year":2022,"lang":"en","type":"article","venue":"Analytical Methods","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of the Fraser Valley","funders":"Natural Sciences and Engineering Research Council of Canada; University of the Fraser Valley","keywords":"Immobilized enzyme; Microreactor; Peptide; Chemistry; Enzyme; Proteolytic enzymes; Peptide mapping; Chromatography; Formaldehyde; Biochemistry; Peptide sequence; Catalysis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.004696399,0.0003812052,0.0007170974,0.0003462039,0.0003969208,0.00004169417,0.0005304537,0.0001777872,0.000133669],"category_scores_gemma":[0.002146467,0.000347462,0.000411616,0.001111798,0.00008014024,0.00002193811,0.0006029613,0.0001896891,0.000001389231],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001519634,"about_ca_system_score_gemma":0.0002882007,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001998273,"about_ca_topic_score_gemma":0.000008590573,"domain_scores_codex":[0.9963135,0.000598683,0.001280717,0.0009016629,0.0003145705,0.0005909397],"domain_scores_gemma":[0.997892,0.0004379944,0.000420124,0.0006859282,0.0003305555,0.0002333683],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005529879,0.0003139874,0.00008381985,0.00008363027,0.0002517677,4.312332e-7,0.0005870776,0.002286156,0.9736246,0.0002630326,0.0004484572,0.02150399],"study_design_scores_gemma":[0.001085558,0.0004018009,0.0002442355,0.0000197483,0.0001378537,0.00001086153,0.0009595394,0.01309268,0.8135682,0.00003670591,0.1700115,0.0004313108],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1678863,0.0002163497,0.8306122,0.00008143778,0.0001731849,0.0008417189,0.00005312047,0.00003010061,0.0001055922],"genre_scores_gemma":[0.09674682,0.00001383774,0.900137,0.0004203927,0.0001003255,0.0009200371,0.0005937212,0.00008179793,0.0009860327],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.169563,"threshold_uncertainty_score":0.9998977,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0846768734235417,"score_gpt":0.4003339392417178,"score_spread":0.3156570658181762,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}