{"id":"W4297180148","doi":"10.1016/j.fsi.2022.09.038","title":"Production of a monoclonal antibody specific to sablefish (Anoplopoma fimbria) IgM and its application in ELISA, western blotting, and immunofluorescent staining","year":2022,"lang":"en","type":"article","venue":"Fish & Shellfish Immunology","topic":"Aquaculture disease management and microbiota","field":"Immunology and Microbiology","cited_by":8,"is_retracted":false,"has_abstract":false,"ca_institutions":"Memorial University of Newfoundland","funders":"Western Regional Aquaculture Center; Washington Sea Grant, University of Washington; National Oceanic and Atmospheric Administration; Canada First Research Excellence Fund; Ocean Frontier Institute; Memorial University of Newfoundland; National Institute of Food and Agriculture; University of Washington","keywords":"Biology; Aeromonas salmonicida; Blot; Antibody; Monoclonal antibody; Western blot; Aquaculture; Immunity; Vaccination; Fishery; Immune system; Immunology; Fish <Actinopterygii>; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007696945,0.0009109257,0.0005201148,0.001031991,0.0003730129,0.0004596686,0.0006128758,0.000612388,0.001934244],"category_scores_gemma":[0.0005378472,0.0004948826,0.0008401069,0.0006385333,0.0002708175,0.0003458674,0.0004554253,0.001213123,0.000974462],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004127527,"about_ca_system_score_gemma":0.0003134138,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00116271,"about_ca_topic_score_gemma":0.00126649,"domain_scores_codex":[0.9995769,0.000058765,0.00006085865,0.00009662191,0.0001018337,0.0001050218],"domain_scores_gemma":[0.9994807,0.0001175028,0.00005849388,0.00008594165,0.0001887133,0.00006867082],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002517349,0.00004464422,0.0001002952,0.00003475112,0.000005887878,0.00001702705,0.000009345372,0.00003115718,0.9983658,0.00004002906,0.00003036645,0.001295564],"study_design_scores_gemma":[0.00004648211,0.0005872825,0.004604336,0.00001715437,0.00007456109,0.0002485512,0.00003360693,0.001374541,0.9859558,0.00009054154,0.006956045,0.00001108922],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.816292,0.003299393,0.1685405,0.0004406757,0.0003419234,0.000938541,0.001783512,0.0008573826,0.007506019],"genre_scores_gemma":[0.8305075,0.003272498,0.1406364,0.0003955693,0.0001935028,0.001165726,0.008872873,0.0001728926,0.01478321],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001934244,"threshold_uncertainty_score":0.006470621,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008904265533874413,"score_gpt":0.2335128327300074,"score_spread":0.224608567196133,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}