{"id":"W4297207040","doi":"10.1101/2022.09.26.509570","title":"Efficient CRISPR/Cas9 mediated large insertions using long single-stranded oligonucleotide donors in <i>C. elegans</i>","year":2022,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children","funders":"","keywords":"CRISPR; Cas9; Oligonucleotide; Biology; Computational biology; DNA; Template; Genetics; Exonuclease; Computer science; Gene; Polymerase","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008009288,0.0005715752,0.0005159948,0.0004635153,0.0004818265,0.0006595197,0.0007775627,0.0006634526,0.002055094],"category_scores_gemma":[0.0004706922,0.0004861694,0.0004686098,0.0002816662,0.0005576172,0.0004307607,0.0008291123,0.001241883,0.001850049],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006594785,"about_ca_system_score_gemma":0.0007799953,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001768136,"about_ca_topic_score_gemma":0.003950119,"domain_scores_codex":[0.9993723,0.00006022023,0.0000761303,0.0001700605,0.0002499516,0.00007136389],"domain_scores_gemma":[0.9997337,0.00004696936,0.00007078079,0.00006110421,0.00003755803,0.00004981775],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003027072,0.00001724515,0.0000818427,0.00005662502,0.000004840428,0.0000516562,0.00001355652,0.0001337276,0.9968587,0.00029552,0.0001992553,0.002256578],"study_design_scores_gemma":[0.000006462721,0.00002978317,0.0002577839,0.00000748927,0.000005706705,0.00009064329,0.00000598106,0.0004727597,0.9951148,0.0000496225,0.003950637,0.000008262565],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6255322,0.001745022,0.3439181,0.000573843,0.0005585802,0.001264137,0.005870676,0.006855139,0.01368227],"genre_scores_gemma":[0.7332419,0.001418694,0.2383481,0.0002845139,0.00003227059,0.0006404188,0.005168726,0.001851492,0.01901395],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002055094,"threshold_uncertainty_score":0.006874919,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0112727304884001,"score_gpt":0.2525419811633304,"score_spread":0.2412692506749303,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}