{"id":"W4297475217","doi":"10.1002/pro.4440","title":"A genetically encoded far‐red fluorescent calcium ion biosensor derived from a biliverdin‐binding protein","year":2022,"lang":"en","type":"article","venue":"Protein Science","topic":"Photoreceptor and optogenetics research","field":"Neuroscience","cited_by":18,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"National Cancer Institute; Japan Society for the Promotion of Science; Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; University of California, San Diego; National Institutes of Health; Toyota Physical and Chemical Research Institute; Hitachi Global Foundation; Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering; National Institute of Diabetes and Digestive and Kidney Diseases; University of Tokyo; Astellas Foundation for Research on Metabolic Disorders; Astellas Pharma; University of Alberta; Montana State University; National Institute of Neurological Disorders and Stroke; Kato Memorial Bioscience Foundation; Japan Agency for Medical Research and Development; Takeda Science Foundation","keywords":"Biliverdin; Fluorescence; Green fluorescent protein; Biophysics; Far-red; Fluorescent protein; Calcium imaging; Protein engineering; In vivo; Chemistry; Calcium; Biochemistry; Biology; Optics; Heme; Genetics; Gene; Physics; Red light; Botany","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001819484,0.0004180759,0.0003299092,0.0002018816,0.0001503632,0.0003618837,0.0005628316,0.0007687521,0.0004861245],"category_scores_gemma":[0.0002427135,0.0001807778,0.0002317335,0.0001801386,0.0003477494,0.0002758295,0.0003721412,0.0006970403,0.0003394699],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009777425,"about_ca_system_score_gemma":0.000423825,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001275322,"about_ca_topic_score_gemma":0.0008324379,"domain_scores_codex":[0.9998344,0.00001591599,0.000008787359,0.0000524828,0.00005313301,0.00003511938],"domain_scores_gemma":[0.9998852,0.00001810381,0.00003206531,0.000007883124,0.00002333277,0.00003340901],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000008618462,0.000004481235,0.00002568643,0.00000902804,8.776715e-7,0.00001258161,0.000003596658,0.00006861666,0.9995271,0.00006275038,0.00001490266,0.0002618427],"study_design_scores_gemma":[0.000005962247,0.00003293631,0.0003090929,0.000002370691,0.000002843625,0.00006667801,0.000006147369,0.001558758,0.9972825,0.00001613788,0.0007101663,0.000006373279],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9616283,0.0005236785,0.03441134,0.0003857248,0.00005964465,0.00007982006,0.0004051348,0.000355517,0.002150783],"genre_scores_gemma":[0.9485068,0.0004125921,0.04612779,0.0001288598,0.000007004926,0.00009189866,0.0005788797,0.00008087468,0.00406536],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001275322,"threshold_uncertainty_score":0.007094026,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06405867834462771,"score_gpt":0.3141888406935224,"score_spread":0.2501301623488947,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}