{"id":"W4300864468","doi":"10.22541/au.166480383.39885458/v1","title":"Strategies to prevent self-ligation of adapters used in enzyme mediated methylation sequencing","year":2022,"lang":"en","type":"preprint","venue":"","topic":"Epigenetics and DNA Methylation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval; Centre hospitalier de l'Université Laval","funders":"","keywords":"Methylation; Ligation; DNA methylation; Restriction enzyme; Computational biology; Biology; Endonuclease; Enzyme; Genetics; Molecular biology; Biochemistry; DNA; Gene; Gene expression","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00239034,0.001326527,0.00093626,0.0006190281,0.0004050912,0.0008547755,0.0008364195,0.001361082,0.0009306734],"category_scores_gemma":[0.003126933,0.000655646,0.0009815617,0.0004245486,0.0006153044,0.0008670705,0.0008793175,0.002432333,0.0009773704],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002269564,"about_ca_system_score_gemma":0.0005693112,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002589505,"about_ca_topic_score_gemma":0.0005565826,"domain_scores_codex":[0.998317,0.0004621152,0.0002571408,0.0003249074,0.0004458998,0.0001929264],"domain_scores_gemma":[0.9976562,0.000660362,0.0006410707,0.0006091559,0.0002977627,0.0001354037],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001298188,0.00006940375,0.0005324129,0.0004023557,0.00005664142,0.0001675777,0.0001401369,0.0002624719,0.9731627,0.001016097,0.0001851959,0.02387522],"study_design_scores_gemma":[0.00001073311,0.0001278032,0.0004588725,0.00001675318,0.00006157876,0.0003280533,0.00001545107,0.0006932928,0.9931162,0.0002040235,0.004946026,0.00002129622],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.3029848,0.006105217,0.6829442,0.0004427806,0.0002612715,0.0008305637,0.0003710071,0.002840594,0.003219571],"genre_scores_gemma":[0.5011511,0.004462732,0.4853865,0.000749112,0.00005512714,0.0007219876,0.001571279,0.0005094804,0.005392624],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.00239034,"threshold_uncertainty_score":0.01264149,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02594814950602012,"score_gpt":0.2956760689326618,"score_spread":0.2697279194266416,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}