{"id":"W4302281956","doi":"10.1101/2022.10.05.510939","title":"Rapid isolation of respiring skeletal muscle mitochondria using nitrogen cavitation","year":2022,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Mitochondrial Function and Pathology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Trent University; Nottingham Trent University","keywords":"Mitochondrion; Bioenergetics; Skeletal muscle; Differential centrifugation; Cytoplasm; Biology; Centrifugation; Respirometry; Cell biology; Biochemistry; Ultracentrifuge; Cell; Biophysics; Chemistry; Anatomy","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008112384,0.000602757,0.0003806874,0.0006483655,0.0004815639,0.0005389634,0.0007592631,0.0005449225,0.001874385],"category_scores_gemma":[0.0006100109,0.0002376421,0.0003567524,0.0003429708,0.0005213781,0.0005196505,0.0006918355,0.001077291,0.001952896],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004712143,"about_ca_system_score_gemma":0.0004339991,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006115778,"about_ca_topic_score_gemma":0.001317149,"domain_scores_codex":[0.9994321,0.00005213307,0.00004555582,0.0001928555,0.0002283326,0.00004903542],"domain_scores_gemma":[0.9996352,0.00008660762,0.00006307337,0.00008300046,0.00009675608,0.00003538231],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002135286,0.00001066164,0.0001297827,0.00006851008,0.000005421152,0.00004417534,0.00002037351,0.00004735274,0.9964758,0.0001686532,0.0001784291,0.002829439],"study_design_scores_gemma":[0.00002298127,0.0001348419,0.003274462,0.00002939271,0.00001711038,0.0006639909,0.00002105062,0.00140686,0.9802142,0.0003429636,0.01385414,0.00001797616],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3552645,0.009956094,0.6160138,0.001179977,0.0005653455,0.001610113,0.003687067,0.003005769,0.00871739],"genre_scores_gemma":[0.4769888,0.007411098,0.4898788,0.0006100736,0.0002480702,0.001624068,0.009148828,0.000965674,0.01312446],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001874385,"threshold_uncertainty_score":0.006270468,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01888757139668228,"score_gpt":0.2439171659977432,"score_spread":0.2250295946010609,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}