{"id":"W4304118523","doi":"10.1002/jev2.12220","title":"Contaminating transfection complexes can masquerade as small extracellular vesicles and impair their delivery of RNA","year":2022,"lang":"en","type":"article","venue":"Journal of Extracellular Vesicles","topic":"Extracellular vesicles in disease","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"The Metabolomics Innovation Centre; University of Alberta; University of Ottawa","funders":"","keywords":"Transfection; Endosome; Small interfering RNA; Microvesicles; RNA; Extracellular; Molecular biology; Cell biology; Biology; Gene delivery; Nucleic acid; Cytoplasm; RNA interference; Chemistry; Biochemistry; Intracellular; microRNA; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.001547851,0.0004122386,0.0005849208,0.0002599155,0.0003852466,0.00006174101,0.0005865157,0.000164588,0.0001013403],"category_scores_gemma":[0.0001414409,0.0004130225,0.0004625912,0.0002336264,0.000320091,0.00003473935,0.0002390581,0.0005388709,0.000001172632],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00008423335,"about_ca_system_score_gemma":0.000278468,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00007171644,"about_ca_topic_score_gemma":0.00003116153,"domain_scores_codex":[0.9966409,0.0006367547,0.001260546,0.0004695086,0.000528702,0.0004635493],"domain_scores_gemma":[0.9977877,0.0001414419,0.00104286,0.0004644004,0.0002643873,0.0002991774],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005172035,0.0004988858,0.003116445,0.0001251966,0.0002639017,0.0002243585,0.0007745317,0.0007336231,0.9775113,0.0003997715,0.0001590005,0.01567577],"study_design_scores_gemma":[0.002000663,0.001908425,0.003415143,0.00008172276,0.0002077898,0.001073996,0.003733447,0.0004855414,0.9789059,0.0008468861,0.006863472,0.000477013],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9526681,0.04399436,0.002285447,0.0002447357,0.0002466362,0.0003390094,0.00008746624,0.00001857945,0.0001156157],"genre_scores_gemma":[0.9963515,0.0008791488,0.001988779,0.00008280096,0.0002966717,0.00001606152,0.00005103084,0.0000873883,0.0002466197],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.04368335,"threshold_uncertainty_score":0.9998322,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01238707818179212,"score_gpt":0.2244073159316884,"score_spread":0.2120202377498963,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}