{"id":"W4304693802","doi":"10.1002/cpz1.571","title":"Methods for Recombinant Production and Purification of Peptides as SUMO‐Peptide‐Intein Fusion Proteins to Protect from Degradation","year":2022,"lang":"en","type":"article","venue":"Current Protocols","topic":"RNA and protein synthesis mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Intein; Fusion protein; Peptide; Escherichia coli; Tandem affinity purification; Biochemistry; Recombinant DNA; Cloning (programming); Target protein; Heterologous expression; Protein purification; Expression vector; Affinity chromatography; Fusion gene; Biology; Chemistry; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001181863,0.0001676293,0.0001982635,0.00008435747,0.0002345039,0.00002883178,0.0002046206,0.00006487625,0.00003325447],"category_scores_gemma":[0.0007937421,0.0001612934,0.00008001611,0.0001364187,0.00002880054,0.00001234264,0.0001759715,0.00009454968,0.000002190651],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003553679,"about_ca_system_score_gemma":0.00009303271,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003255059,"about_ca_topic_score_gemma":0.000006492998,"domain_scores_codex":[0.9983078,0.0004120164,0.0003635398,0.0005646622,0.0001717433,0.0001802834],"domain_scores_gemma":[0.9990608,0.00002482332,0.0002827598,0.0004142885,0.0001494171,0.0000679293],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004758903,0.0001435569,0.00006580558,0.0001138098,0.00001210425,4.685803e-8,0.00008796776,0.00001078728,0.7644398,0.0002145421,0.0002678849,0.2341678],"study_design_scores_gemma":[0.0003326465,0.001455314,0.0001999846,0.0001416437,0.00001337905,0.000002577705,0.00007752706,0.00003914734,0.8709564,0.002922423,0.1236825,0.0001765326],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"protocol","genre_scores_codex":[0.5029509,0.0005059346,0.2296083,0.0008377364,0.0004820154,0.2654205,0.00009607178,0.00004588802,0.00005258369],"genre_scores_gemma":[0.1113339,0.00002729228,0.1343284,0.00003341756,0.0002384405,0.7530515,0.000312605,0.0000517646,0.0006227577],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.4876309,"threshold_uncertainty_score":0.6577354,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0532364194301609,"score_gpt":0.3778106212054986,"score_spread":0.3245742017753377,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}