{"id":"W4306353454","doi":"10.1101/2022.10.15.512367","title":"SLICER: Seamless Loss of Integrated Cassettes Using Endonuclease Cleavage and Recombination in <i>Deinococcus radiodurans</i>","year":2022,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Deinococcus radiodurans; Selectable marker; Genome; Endonuclease; Genome editing; Restriction enzyme; Computational biology; Biology; Transformation (genetics); Gene; DNA; Genetics; Homing endonuclease","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005968121,0.001081344,0.0006312396,0.0009677007,0.0002399203,0.000891052,0.0007755499,0.0005797664,0.02629501],"category_scores_gemma":[0.0005334925,0.000550957,0.000820077,0.0008897443,0.0003342904,0.0006704141,0.0006648634,0.002005877,0.008795751],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000448353,"about_ca_system_score_gemma":0.0004928621,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008260388,"about_ca_topic_score_gemma":0.001095178,"domain_scores_codex":[0.9995462,0.0000503271,0.00002339633,0.000106364,0.0002106843,0.00006302194],"domain_scores_gemma":[0.9997967,0.00004537049,0.00006372304,0.00003663622,0.00002288641,0.000034619],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005636344,0.0001173284,0.0009704843,0.001244987,0.0001564391,0.0007484732,0.000137424,0.002157955,0.8935029,0.006856848,0.03060032,0.06294313],"study_design_scores_gemma":[0.000104367,0.0001458415,0.001874885,0.00006697299,0.00005210513,0.0005354251,0.00004083616,0.003433354,0.9075094,0.0009250232,0.08525988,0.0000518443],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.2833345,0.006367006,0.5134376,0.00429531,0.002522337,0.001003726,0.0768133,0.06227679,0.04994952],"genre_scores_gemma":[0.5886994,0.005480487,0.2867865,0.0007575506,0.0001623733,0.0006651261,0.05073614,0.00613705,0.0605753],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.02629501,"threshold_uncertainty_score":0.08796555,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00879611802290653,"score_gpt":0.2470596082501871,"score_spread":0.2382634902272805,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}