{"id":"W4307460328","doi":"10.1016/j.xpro.2022.101853","title":"Characterization of multiple interactions between the envelope E protein of SARS-CoV-2 and human BRD4","year":2022,"lang":"en","type":"article","venue":"STAR Protocols","topic":"Protein Degradation and Inhibitors","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval","funders":"National Institute of Allergy and Infectious Diseases; National Institute of General Medical Sciences; National Institute on Drug Abuse; National Heart, Lung, and Blood Institute; Canadian Institutes of Health Research; National Institute on Aging; National Cancer Institute; National Institutes of Health","keywords":"Bromodomain; BRD4; Protein–protein interaction; Computational biology; Nuclear magnetic resonance spectroscopy; Acetylation; Cell biology; Chemistry; Biology; Virology; Biophysics; Genetics; Stereochemistry; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001989045,0.00007015613,0.0001002925,0.00003350813,0.000140758,0.00000853979,0.0001332153,0.00002687888,0.00002112128],"category_scores_gemma":[0.00003783794,0.00005848075,0.000035749,0.00008089485,0.00006849968,0.000006353173,0.0001664651,0.0000825582,4.802031e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000008497669,"about_ca_system_score_gemma":0.0000387089,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003089672,"about_ca_topic_score_gemma":0.00001085089,"domain_scores_codex":[0.9993168,0.0001171574,0.0002379714,0.0001390018,0.0001121706,0.00007687498],"domain_scores_gemma":[0.9995108,0.000005870927,0.0002066843,0.0002072665,0.00005661904,0.0000128011],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000391751,0.0000428416,0.003386082,0.00003086352,0.00001707354,8.388203e-8,0.00006044333,3.296474e-7,0.9954209,0.0001033722,0.0001163671,0.000782482],"study_design_scores_gemma":[0.0003330754,0.0002946116,0.007361222,0.00001780386,0.000003317335,7.563576e-7,0.00003384413,0.000004747627,0.9074088,0.00004702826,0.08443559,0.00005916496],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9762738,0.000003257848,0.0003062045,0.0001208455,0.00001250365,0.02298289,0.0001179896,0.000005385528,0.0001771299],"genre_scores_gemma":[0.9681585,3.914637e-7,0.0001810472,0.00005543075,0.00004983184,0.03116328,0.0002000356,0.00001017029,0.0001813553],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.08801205,"threshold_uncertainty_score":0.2384776,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03765331847517312,"score_gpt":0.3215858308150582,"score_spread":0.2839325123398851,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}