{"id":"W4308981342","doi":"10.1016/j.snb.2022.132992","title":"A red fluorescent small-molecule for visualization of higher-order cyclic dimeric guanosine monophosphate (c-di-GMP) structure in live bacterial cells and real-time monitoring of biofilm formation on biotic and abiotic surfaces","year":2022,"lang":"en","type":"article","venue":"Sensors and Actuators B Chemical","topic":"Bacterial biofilms and quorum sensing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":false,"ca_institutions":"Lakehead University; Thunder Bay Regional Research Institute","funders":"Health and Medical Research Fund; Department of Education of Guangdong Province; Guangdong University of Technology; Research Grants Council, University Grants Committee; Hong Kong Polytechnic University; Natural Science Foundation of Guangdong Province; Department of Agriculture of Guangdong Province; National Natural Science Foundation of China; Institut National de la Santé et de la Recherche Médicale","keywords":"Biofilm; Tetramer; Chemistry; Dimer; Biophysics; Guanosine; Bacteria; Fluorescence; Guanosine monophosphate; Monomer; FtsZ; Biochemistry; Nucleotide; Stereochemistry; Biology; Cell; Cell division","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003100215,0.0004079056,0.0002388416,0.0002709853,0.0002795394,0.0002382444,0.0005788181,0.0008533628,0.001791256],"category_scores_gemma":[0.0001745846,0.0002562675,0.0002174683,0.0001646671,0.0003001464,0.0003022726,0.0003237675,0.0007917303,0.0004841752],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004080106,"about_ca_system_score_gemma":0.0002739506,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004570673,"about_ca_topic_score_gemma":0.0006582105,"domain_scores_codex":[0.999872,0.00002755622,0.000005208251,0.00003530557,0.00003307185,0.00002689971],"domain_scores_gemma":[0.9998572,0.00004946319,0.00002077938,0.00001742991,0.00001321129,0.00004197306],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003543843,0.00001247981,0.00001854775,0.00003181526,0.000001990863,0.00003164719,0.000006777678,0.00004019187,0.9980978,0.0002747292,0.0001160135,0.001332512],"study_design_scores_gemma":[0.00001675001,0.0000963155,0.0004055319,0.000003158299,0.00000578135,0.0001691432,0.00000492465,0.001130429,0.9954602,0.00004734402,0.00265462,0.000005792957],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8555182,0.007228705,0.1220944,0.001457762,0.0003460679,0.0002451312,0.0007578552,0.001471706,0.01088021],"genre_scores_gemma":[0.8800114,0.002800342,0.106033,0.0006916744,0.00007507463,0.0002898359,0.0006223363,0.00009094858,0.009385368],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001791256,"threshold_uncertainty_score":0.005992413,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006692681666030846,"score_gpt":0.2230672511153893,"score_spread":0.2163745694493585,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}