{"id":"W4309330402","doi":"10.1007/978-1-0716-2772-3_11","title":"Morpholino-Mediated Exons 28–29 Skipping of Dysferlin and Characterization of Multiexon-skipped Dysferlin using RT-PCR, Immunoblotting, and Membrane Wounding Assay","year":2022,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Muscle Physiology and Disorders","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":false,"ca_institutions":"Muscular Dystrophy Canada; University of Alberta","funders":"Canadian Institutes of Health Research","keywords":"Dysferlin; Morpholino; Limb-girdle muscular dystrophy; Exon; Myopathy; Muscular dystrophy; Exon skipping; Molecular biology; Biology; Phenotype; Genetics; Cell biology; Gene; Alternative splicing; Gene knockdown","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004564743,0.0009942089,0.0003920035,0.0007100543,0.000454814,0.0004414239,0.0005396316,0.0004963923,0.002112726],"category_scores_gemma":[0.0003181105,0.0005232979,0.0008093836,0.0003636161,0.0006173457,0.0003833018,0.0004138357,0.001397746,0.001203174],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005767744,"about_ca_system_score_gemma":0.0003146641,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008106765,"about_ca_topic_score_gemma":0.002222637,"domain_scores_codex":[0.9994791,0.00006726862,0.00007495016,0.0001664971,0.0001286228,0.00008357126],"domain_scores_gemma":[0.9994199,0.0001852492,0.0001780765,0.00008460298,0.00005750482,0.00007455819],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002386265,0.00001383988,0.0000524149,0.0000189817,0.000003950619,0.00004921581,0.00002317983,0.00002953871,0.9993621,0.0001163882,0.00003033247,0.0002763682],"study_design_scores_gemma":[0.000009583077,0.00004847803,0.002128501,0.000005815114,0.00001652173,0.0002416426,0.00002791843,0.0007263367,0.9944744,0.00005626871,0.002257328,0.000007279778],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9112257,0.001454519,0.07765305,0.0004444418,0.0002426271,0.000529321,0.003840625,0.0007126823,0.003896977],"genre_scores_gemma":[0.8741204,0.001783011,0.09317593,0.0003841323,0.00005473741,0.0007929956,0.01168253,0.0005340605,0.01747219],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002112726,"threshold_uncertainty_score":0.00706774,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01850937250168814,"score_gpt":0.3349659116414916,"score_spread":0.3164565391398034,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}