{"id":"W4309412933","doi":"10.17912/micropub.biology.000657","title":"A new combinatorial megaplasmid library assembly method designed to screen for minimal pathways by using SCRaMbLE","year":2022,"lang":"en","type":"article","venue":"PubMed","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"","keywords":"Complementary DNA; Computational biology; Streptavidin; cDNA library; Yeast; Biology; Genomic library; Saccharomyces cerevisiae; Synthetic biology; Gene; Genetics; Base sequence; Biotin","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005075688,0.001218335,0.0009068953,0.001178607,0.0004952722,0.000725476,0.0008905513,0.0005924959,0.001846495],"category_scores_gemma":[0.0004033365,0.0005705938,0.0007041952,0.0006554536,0.0003617799,0.0004950898,0.0008271734,0.001702003,0.001260861],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004226144,"about_ca_system_score_gemma":0.0005848659,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004120329,"about_ca_topic_score_gemma":0.001188782,"domain_scores_codex":[0.9993373,0.00008093248,0.00007147472,0.00020171,0.0002490088,0.00005958914],"domain_scores_gemma":[0.9996721,0.00006455857,0.00007257968,0.00005925651,0.00005507838,0.00007655092],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004835034,0.00003620742,0.0001519789,0.0001117436,0.00001921749,0.00007080122,0.00002473711,0.0001446888,0.9909284,0.0003565206,0.0002369772,0.007870457],"study_design_scores_gemma":[0.00002323576,0.0001853063,0.0008924971,0.000006732572,0.00004411089,0.0005281273,0.00001058075,0.002794864,0.9858371,0.0001063599,0.009544117,0.000027065],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.141812,0.001554717,0.8463945,0.0002577742,0.0002105694,0.0007665201,0.00174723,0.004046505,0.003210222],"genre_scores_gemma":[0.233395,0.001821904,0.7446465,0.0003606301,0.00004468089,0.001093716,0.009695423,0.0007890475,0.008153101],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001846495,"threshold_uncertainty_score":0.006177127,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01955335042577365,"score_gpt":0.2767560750601619,"score_spread":0.2572027246343883,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}