{"id":"W4309437992","doi":"10.1038/s41467-022-34269-7","title":"High-affinity chromodomains engineered for improved detection of histone methylation and enhanced CRISPR-based gene repression","year":2022,"lang":"en","type":"article","venue":"Nature Communications","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"National Institute of General Medical Sciences; National Cancer Institute","keywords":"Chromatin; Histone; Computational biology; Biology; Gene silencing; Epigenetics; Regulation of gene expression; CRISPR; Genetics; Cell biology; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002879427,0.0004102677,0.000247837,0.0002027545,0.0001357334,0.0003721682,0.0004246176,0.0004683107,0.0008101869],"category_scores_gemma":[0.0002423944,0.0002060811,0.0002062432,0.0001637549,0.0003815839,0.0002227523,0.0003933209,0.0007333569,0.0002749882],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006288023,"about_ca_system_score_gemma":0.0002248876,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007989709,"about_ca_topic_score_gemma":0.001590251,"domain_scores_codex":[0.999725,0.00003360726,0.00001665188,0.00006288923,0.00009900491,0.00006273199],"domain_scores_gemma":[0.9998446,0.00003602554,0.00004399489,0.00001685369,0.00001966757,0.00003888468],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001469854,0.00001124962,0.00006285351,0.00001418001,0.000003377864,0.00002593417,0.000007792763,0.0001489755,0.9988846,0.000151162,0.00002787498,0.0006474694],"study_design_scores_gemma":[0.000005176243,0.00003453619,0.0003678529,0.000001060535,0.000003216008,0.00005742058,0.000004525697,0.001321288,0.9972606,0.00002594017,0.0009139297,0.000004369496],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9368176,0.0009754082,0.05905632,0.0001896152,0.00006343287,0.00008724407,0.0002670613,0.0004865427,0.002056809],"genre_scores_gemma":[0.9760969,0.0003743603,0.02022949,0.0001226417,0.000007439933,0.00004262275,0.0002743134,0.00006289977,0.002789327],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008101869,"threshold_uncertainty_score":0.004562259,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009336897012646266,"score_gpt":0.3322601715175582,"score_spread":0.3229232745049119,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}