{"id":"W4309437992","doi":"10.1038/s41467-022-34269-7","title":"High-affinity chromodomains engineered for improved detection of histone methylation and enhanced CRISPR-based gene repression","year":2022,"lang":"en","type":"article","venue":"Nature Communications","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"National Institute of General Medical Sciences; National Cancer Institute","keywords":"Chromatin; Histone; Computational biology; Biology; Gene silencing; Epigenetics; Regulation of gene expression; CRISPR; Genetics; Cell biology; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001997805,0.00008519505,0.0001069259,0.00005978895,0.0002245437,0.000004756123,0.0002336382,0.0001149025,0.000002783916],"category_scores_gemma":[0.0001642283,0.00009655464,0.0000549763,0.0001076004,0.0000349734,0.000002727558,0.00018136,0.0002217498,5.537609e-8],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002911225,"about_ca_system_score_gemma":0.0000320383,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000148857,"about_ca_topic_score_gemma":0.00006313308,"domain_scores_codex":[0.999447,0.00006459847,0.0001659537,0.0001736892,0.00006033136,0.00008838323],"domain_scores_gemma":[0.998943,0.0000619344,0.00008990373,0.0007759118,0.00009974733,0.00002944357],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005825457,0.00005308825,0.000004082737,0.00001267928,0.00001934959,1.678819e-8,0.00003385897,0.003979909,0.9930566,0.0000544734,0.00006168473,0.002666006],"study_design_scores_gemma":[0.0005803409,0.0001763575,0.001129369,0.000003001132,0.000029362,0.000001395778,0.00002444012,0.009409223,0.9828737,0.00004062171,0.005632656,0.00009951503],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6292772,0.006510988,0.3629101,0.0004337753,0.0002022338,0.0004641046,0.0001490318,0.00002718105,0.00002535999],"genre_scores_gemma":[0.9709898,0.0001812161,0.02795229,0.00004869818,0.00003392051,0.0001872991,0.0005659698,0.00001642483,0.00002437541],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.3417126,"threshold_uncertainty_score":0.3937384,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009336897012646266,"score_gpt":0.3322601715175582,"score_spread":0.3229232745049119,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}