{"id":"W4309857868","doi":"10.3390/biomedicines10123028","title":"Coupling CRISPR/Cas9 and Lambda Red Recombineering System for Genome Editing of Salmonella Gallinarum and the Effect of ssaU Knock-Out Mutant on the Virulence of Bacteria","year":2022,"lang":"en","type":"article","venue":"Biomedicines","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"International Development Research Centre","keywords":"Virulence; Biology; CRISPR; Salmonella; Microbiology; Recombineering; Type three secretion system; Pathogenicity island; Plasmid; Genome; Mutant; Genome editing; Effector; Cas9; Genomic island; Genetics; Gene; Bacteria; Cell biology","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002242814,0.0004477203,0.0003584218,0.0002299842,0.0001580163,0.0003272647,0.0003608672,0.0005921902,0.001239333],"category_scores_gemma":[0.0002075213,0.0002428086,0.0003992213,0.0001251209,0.0002679701,0.000165837,0.0003402538,0.000710671,0.0003223246],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002287571,"about_ca_system_score_gemma":0.0002041979,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001117965,"about_ca_topic_score_gemma":0.001339238,"domain_scores_codex":[0.9997215,0.00003314068,0.00003931292,0.00008292068,0.00007596185,0.00004723362],"domain_scores_gemma":[0.9998615,0.00002265017,0.00005137351,0.00001937336,0.00001358336,0.00003148899],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006457778,0.00003242143,0.00008605589,0.00007009289,0.000007441989,0.0001104727,0.00002587383,0.0001558265,0.9980299,0.0001145381,0.00006114294,0.001241527],"study_design_scores_gemma":[0.00002385391,0.0003654911,0.001546912,0.00001258294,0.00002732601,0.0003470998,0.00003516907,0.002782357,0.9907221,0.00005600115,0.004065065,0.00001616849],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9698977,0.001485086,0.02303374,0.0002262485,0.00015183,0.0001948454,0.0008112558,0.000719819,0.003479589],"genre_scores_gemma":[0.9795859,0.0007132809,0.01361141,0.00008367837,0.000009509708,0.0001287804,0.000650975,0.00007739227,0.005139106],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001239333,"threshold_uncertainty_score":0.00414598,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006331790869373532,"score_gpt":0.2521249196048188,"score_spread":0.2457931287354452,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}