{"id":"W4309857868","doi":"10.3390/biomedicines10123028","title":"Coupling CRISPR/Cas9 and Lambda Red Recombineering System for Genome Editing of Salmonella Gallinarum and the Effect of ssaU Knock-Out Mutant on the Virulence of Bacteria","year":2022,"lang":"en","type":"article","venue":"Biomedicines","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"International Development Research Centre","keywords":"Virulence; Biology; CRISPR; Salmonella; Microbiology; Recombineering; Type three secretion system; Pathogenicity island; Plasmid; Genome; Mutant; Genome editing; Effector; Cas9; Genomic island; Genetics; Gene; Bacteria; Cell biology","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007824191,0.0001152069,0.0002607146,0.00004259647,0.0000786889,0.00000390384,0.0001239864,0.00003797562,0.000002303395],"category_scores_gemma":[0.0001197271,0.00006949014,0.00005139232,0.00006961407,0.0001383382,0.000001228468,0.0001181739,0.00005326476,2.85498e-8],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000006728958,"about_ca_system_score_gemma":0.000009416934,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001773811,"about_ca_topic_score_gemma":0.000001412538,"domain_scores_codex":[0.9992846,0.00004031682,0.0002762838,0.0001652257,0.0001112945,0.0001223154],"domain_scores_gemma":[0.9993807,0.0002073857,0.0001497984,0.0001988179,0.00003876206,0.00002452307],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003294083,0.000006443411,0.0004753162,0.0005954119,0.00005548847,6.177854e-7,0.0001645491,0.0004641246,0.9975958,0.00003154589,0.00002994438,0.0002513709],"study_design_scores_gemma":[0.001890746,0.001725839,0.00136733,0.0002222896,0.00009729447,0.00002865103,0.001324273,0.006317919,0.9859585,0.0000071292,0.0009464136,0.0001136711],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9952701,0.002467485,0.001277262,0.0001825104,0.0003208923,0.0004073274,0.00004564769,0.000005260409,0.00002349006],"genre_scores_gemma":[0.9994941,0.0001668371,0.00008166661,0.000007129009,0.0001523691,0.00005270536,0.00001550644,0.00001493942,0.0000147014],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01163734,"threshold_uncertainty_score":0.2833726,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006331790869373532,"score_gpt":0.2521249196048188,"score_spread":0.2457931287354452,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}