{"id":"W4310539748","doi":"10.1016/j.xpro.2022.101894","title":"In vitro and in cellulo ApoE particle formation, isolation, and characterization","year":2022,"lang":"en","type":"article","venue":"STAR Protocols","topic":"Lipid Membrane Structure and Behavior","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Ontario Institute for Regenerative Medicine; Universität Zürich; Université de Genève; Eidgenössische Technische Hochschule Zürich; Louis-Jeantet Foundation; European Molecular Biology Laboratory; AbbVie","keywords":"Particle (ecology); In vitro; Scattering; Immunoprecipitation; Apolipoprotein E; Membrane; Characterization (materials science); Dynamic light scattering; Chemistry; Biophysics; Light scattering; Fatty acid; Isolation (microbiology); Chemical engineering; Chromatography; Biochemistry; Chemical physics; Materials science; Nanotechnology; Gene; Physics; Optics; Biology; Bioinformatics; Nanoparticle; Engineering","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009329226,0.0008174505,0.0003520372,0.0004195661,0.0004854061,0.0009037111,0.0006284982,0.0004753969,0.001847175],"category_scores_gemma":[0.0004905098,0.0003620876,0.0004480371,0.0006074944,0.0003116897,0.0003707109,0.0004854722,0.001059997,0.002342986],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009266598,"about_ca_system_score_gemma":0.0004770187,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003263385,"about_ca_topic_score_gemma":0.005571481,"domain_scores_codex":[0.9990751,0.0001716872,0.0001283435,0.00016739,0.0003534709,0.0001040216],"domain_scores_gemma":[0.9996766,0.00005742471,0.00006886385,0.00009778282,0.00006369327,0.0000357231],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.00005764667,0.00006385762,0.0002231902,0.00004774913,0.000006021271,0.00005023675,0.00004252338,0.0001799483,0.9967457,0.0004010444,0.0003466553,0.001835292],"study_design_scores_gemma":[0.000006252053,0.00007490513,0.001537349,0.00000730553,0.00001082623,0.00008644704,0.00002060878,0.0007556426,0.9844247,0.00008764341,0.01297908,0.000009204552],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.7026904,0.003535685,0.2288813,0.001054003,0.0004703278,0.00129296,0.03601021,0.001421231,0.02464371],"genre_scores_gemma":[0.5297937,0.007035144,0.3024869,0.000680335,0.0001171879,0.002505675,0.08750243,0.001072576,0.06880608],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.003263385,"threshold_uncertainty_score":0.006723404,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01001569814673187,"score_gpt":0.260146938144888,"score_spread":0.2501312399981561,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}