{"id":"W4311144984","doi":"10.1016/j.mimet.2022.106648","title":"Efficiency of CRISPR-Cas9 genetic engineering in Escherichia coli BL21 is impaired by lack of Lon protease","year":2022,"lang":"en","type":"article","venue":"Journal of Microbiological Methods","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada","funders":"","keywords":"CRISPR; Escherichia coli; Cas9; Protease; Biology; Genome engineering; Computational biology; Protein engineering; Genetics; Gene; Biochemistry; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001089685,0.0001570236,0.0004070926,0.00009572782,0.00002367489,0.0000041705,0.0003156357,0.0001222332,0.00007523862],"category_scores_gemma":[0.0002877894,0.0001334946,0.0002046548,0.0002271941,0.00005468397,0.000002264034,0.0001826031,0.0002810903,1.989579e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002779497,"about_ca_system_score_gemma":0.00006221727,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000009714046,"about_ca_topic_score_gemma":3.372394e-7,"domain_scores_codex":[0.9984812,0.000304304,0.0006979924,0.0001907087,0.0001007732,0.00022499],"domain_scores_gemma":[0.9992712,0.00006298997,0.0003274142,0.0001799014,0.00008609686,0.00007238055],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001416497,0.0002023201,0.001141823,0.00004439049,0.00004056193,0.000006365408,0.00007551935,0.007591174,0.9884852,0.000001835937,0.0005033917,0.001765806],"study_design_scores_gemma":[0.0007689373,0.001646405,0.003194183,0.00002195811,0.00002472401,0.00007243966,0.00008109187,0.0005345651,0.9898275,0.00001777781,0.0036625,0.000147987],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9063841,0.004698607,0.08853875,0.00005476837,0.0001121976,0.00016505,0.00002876526,0.00000270572,0.00001502499],"genre_scores_gemma":[0.9074652,0.0001754319,0.09220455,0.00006103016,0.00004167635,0.000008129798,0.00000445466,0.00001395644,0.00002558352],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.007056608,"threshold_uncertainty_score":0.5443751,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02153646751490054,"score_gpt":0.3578596033018124,"score_spread":0.3363231357869119,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}