{"id":"W4311328706","doi":"10.1016/j.xpro.2022.101917","title":"Quantification of protein enrichment at site-specific DNA double-strand breaks by chromatin immunoprecipitation in cultured human cells","year":2022,"lang":"en","type":"article","venue":"STAR Protocols","topic":"DNA Repair Mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Institute of Population and Public Health; Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Cancer Research Society","keywords":"Immunoprecipitation; Chromatin immunoprecipitation; Chromatin; DNA; Molecular biology; Biology; Cell biology; Chemistry; Genetics; Gene; Promoter; Gene expression","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001297874,0.001322626,0.0009566731,0.001911739,0.0006965688,0.0009664205,0.001053093,0.0008164825,0.003808765],"category_scores_gemma":[0.0005918358,0.0008065305,0.0007281531,0.002039562,0.0004963885,0.000474113,0.000757596,0.00158266,0.00362878],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006023246,"about_ca_system_score_gemma":0.000430193,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001121079,"about_ca_topic_score_gemma":0.002418808,"domain_scores_codex":[0.9980738,0.0002409041,0.0001667628,0.000783558,0.0005732354,0.0001616956],"domain_scores_gemma":[0.9994562,0.0001591365,0.0000604153,0.0001160526,0.0001580205,0.00005021738],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007594394,0.00004537623,0.0004396302,0.0001505535,0.00002759727,0.00003339028,0.00005151204,0.00009507072,0.995421,0.0001700125,0.000241079,0.003248714],"study_design_scores_gemma":[0.00001585543,0.00010772,0.004978023,0.00002627205,0.00005555575,0.0001153649,0.00002383904,0.001605571,0.984611,0.0001526433,0.008294271,0.00001401403],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2619423,0.01995741,0.6776724,0.0004898884,0.000637095,0.001340294,0.02215216,0.004647436,0.01116097],"genre_scores_gemma":[0.3315864,0.01218868,0.591613,0.0006701126,0.0002559208,0.004411462,0.03276145,0.00116876,0.02534416],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003808765,"threshold_uncertainty_score":0.01274157,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0164298581695803,"score_gpt":0.2769167325721786,"score_spread":0.2604868744025983,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}