{"id":"W4312067110","doi":"10.1007/978-1-0716-2895-9_5","title":"Viral Quantification in Bee Samples Using Synthetic DNA Sequences with Real-Time PCR (qPCR)","year":2022,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Insect and Pesticide Research","field":"Agricultural and Biological Sciences","cited_by":4,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Guelph","funders":"","keywords":"Deformed wing virus; Virology; Biology; Virus; Honey bee; Real-time polymerase chain reaction; Pollinator; RNA virus; Pathogen; Microbiology; Genome; Genetics; Gene; Zoology; Ecology; Pollination; Varroa destructor; Pollen","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.004490211,0.004509619,0.002628866,0.002456648,0.002034429,0.002807144,0.002314767,0.003002765,0.00838916],"category_scores_gemma":[0.004050023,0.002638327,0.002708974,0.002246459,0.003693139,0.002145525,0.001190733,0.00610871,0.004790391],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.002162865,"about_ca_system_score_gemma":0.00262075,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002062131,"about_ca_topic_score_gemma":0.005065263,"domain_scores_codex":[0.9907102,0.001743231,0.0008858248,0.003829573,0.002007905,0.0008232083],"domain_scores_gemma":[0.9971486,0.001099314,0.0003059415,0.0005201427,0.0008018642,0.000124095],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002549108,0.0002522108,0.0009594149,0.0005312058,0.00005653171,0.00003751829,0.000292281,0.001194633,0.987682,0.001596914,0.0004927053,0.0066496],"study_design_scores_gemma":[0.00005780216,0.0004392786,0.001401581,0.0001373427,0.00009622469,0.00007412643,0.0001007881,0.01088344,0.9788377,0.0009072785,0.007005681,0.00005871219],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1717819,0.002172827,0.7925865,0.000730295,0.001195742,0.003620135,0.01355012,0.004921116,0.009441423],"genre_scores_gemma":[0.1210974,0.001565533,0.8338708,0.001321113,0.0001614447,0.008950765,0.01718908,0.001332685,0.01451119],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.00838916,"threshold_uncertainty_score":0.02806449,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.08714772426773115,"score_gpt":0.3906365242914798,"score_spread":0.3034888000237487,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}