{"id":"W4312102155","doi":"10.26434/chemrxiv-2022-q1hf6-v2","title":"Minimizing Product Inhibition in DNA Self-Replication: Insights for Prebiotic Replication from the Role of the Enzyme in Lesion-Induced DNA Amplification","year":2022,"lang":"en","type":"preprint","venue":"ChemRxiv","topic":"DNA and Nucleic Acid Chemistry","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; Canada First Research Excellence Fund; Alberta Innovates; Technische Universität München","keywords":"DNA ligase; Okazaki fragments; Isothermal titration calorimetry; DNA; Biochemistry; DNA replication; Nucleic acid; Chemistry; Polymerase; Enzyme; Biophysics; Biology; Eukaryotic DNA replication","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007758359,0.0008020186,0.000938322,0.0003630203,0.0002718597,0.0009098051,0.0007463486,0.0008377,0.001113695],"category_scores_gemma":[0.0008255808,0.0004031302,0.0006016177,0.00019198,0.000779772,0.00167027,0.0004056041,0.001454718,0.000478578],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007274658,"about_ca_system_score_gemma":0.0005542952,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003804349,"about_ca_topic_score_gemma":0.0004506054,"domain_scores_codex":[0.9996843,0.00005421862,0.00002430094,0.00007317843,0.0000891316,0.00007485268],"domain_scores_gemma":[0.9996185,0.0001513677,0.00009060042,0.00003478623,0.0000610071,0.00004374437],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008359716,0.0001732021,0.0008038269,0.0003719159,0.00001095528,0.000134818,0.00007131512,0.002617191,0.9777277,0.004779871,0.00008059289,0.01314513],"study_design_scores_gemma":[0.00001547312,0.0004043965,0.001504221,0.00003132347,0.00001787988,0.0003395405,0.0000651064,0.01799252,0.9724391,0.003592679,0.003563177,0.00003467652],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8064805,0.03434858,0.1505441,0.002077805,0.0001601208,0.0001492645,0.0001824837,0.000567859,0.005489437],"genre_scores_gemma":[0.9611135,0.007105168,0.02950606,0.0002335088,0.00004063157,0.0000713381,0.00009559575,0.00006655698,0.001767539],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001113695,"threshold_uncertainty_score":0.005278111,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02086215064956419,"score_gpt":0.2565715014689094,"score_spread":0.2357093508193452,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}