{"id":"W4312815027","doi":"10.21769/bioprotoc.4560","title":"Assessing the in vitro Binding Affinity of Protein–RNA Interactions Using an RNA Pull-down Technique","year":2022,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"RNA Research and Splicing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"Carleton University","funders":"","keywords":"RNA; Biotinylation; Nucleic acid; RNase P; Agarose; Streptavidin; In vitro; Chemistry; Recombinant DNA; Biochemistry; Molecular biology; Computational biology; Biology; Biophysics; Biotin; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001523899,0.002471187,0.001307437,0.002246625,0.000766322,0.0007638231,0.001117294,0.001057429,0.03289608],"category_scores_gemma":[0.001402122,0.001102803,0.001566427,0.002241179,0.0004667188,0.0005785572,0.0006343963,0.003660799,0.0157655],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007487343,"about_ca_system_score_gemma":0.0008680013,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001630535,"about_ca_topic_score_gemma":0.004223049,"domain_scores_codex":[0.9983271,0.0002583856,0.0001279642,0.0004949061,0.0005239291,0.0002677701],"domain_scores_gemma":[0.9990587,0.000429927,0.00009478057,0.00011601,0.0002185587,0.00008210776],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003697728,0.0001589537,0.0002907731,0.001016731,0.0001096093,0.00007214589,0.00005768589,0.0003997942,0.9801759,0.0006063079,0.006178756,0.01056352],"study_design_scores_gemma":[0.0001149469,0.0004264178,0.002991717,0.0001070334,0.0002094815,0.0001780429,0.00006814615,0.002654419,0.9644855,0.0004627265,0.02822161,0.00007997023],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.4081646,0.0178747,0.3471197,0.004553283,0.00441927,0.00339926,0.1588519,0.01188314,0.0437342],"genre_scores_gemma":[0.3647742,0.01431872,0.3784917,0.001514198,0.0002881039,0.004855616,0.1558227,0.002404348,0.07753046],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.03289608,"threshold_uncertainty_score":0.1100483,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05332558546337698,"score_gpt":0.3993321178272455,"score_spread":0.3460065323638686,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}