{"id":"W4312815027","doi":"10.21769/bioprotoc.4560","title":"Assessing the in vitro Binding Affinity of Protein–RNA Interactions Using an RNA Pull-down Technique","year":2022,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"RNA Research and Splicing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"Carleton University","funders":"","keywords":"RNA; Biotinylation; Nucleic acid; RNase P; Agarose; Streptavidin; In vitro; Chemistry; Recombinant DNA; Biochemistry; Molecular biology; Computational biology; Biology; Biophysics; Biotin; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000836238,0.0001277714,0.0001328355,0.0001104254,0.0003714771,0.00007469964,0.0003616783,0.00006176517,0.00006669459],"category_scores_gemma":[0.00009438741,0.0001033703,0.00007618798,0.0003071242,0.00007447586,0.00002326264,0.0003556445,0.0003566923,9.468849e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00008694276,"about_ca_system_score_gemma":0.0002151353,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000355666,"about_ca_topic_score_gemma":0.00003612227,"domain_scores_codex":[0.9985435,0.0003613083,0.0002925392,0.0002834322,0.0002272502,0.0002919545],"domain_scores_gemma":[0.9992949,0.00001714686,0.0001668596,0.0003976614,0.00006176969,0.0000616351],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001882815,0.0001777733,0.0003007396,0.00003879825,0.00001045436,0.000005405918,0.00002585611,0.00009802576,0.9961734,0.000009393155,0.00003271755,0.002939133],"study_design_scores_gemma":[0.0004722964,0.0002221917,0.0002367208,0.00003407516,0.000002549529,0.00001694921,0.0004949585,0.0008739682,0.9918146,0.00001971488,0.005687776,0.0001241657],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7930671,0.000002339758,0.00114223,0.000065997,0.00002202353,0.2052307,0.00001571477,0.00001279213,0.0004411206],"genre_scores_gemma":[0.5653335,9.879226e-8,0.001288186,0.00002207141,0.0000529626,0.4331976,0.00001295372,0.00001451418,0.00007812525],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2279669,"threshold_uncertainty_score":0.4215319,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05332558546337698,"score_gpt":0.3993321178272455,"score_spread":0.3460065323638686,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}