{"id":"W4315782602","doi":"10.1038/s41467-022-35755-8","title":"Structural basis of transcription recognition of a hydrophobic unnatural base pair by T7 RNA polymerase","year":2023,"lang":"en","type":"article","venue":"Nature Communications","topic":"DNA and Nucleic Acid Chemistry","field":"Biochemistry, Genetics and Molecular Biology","cited_by":21,"is_retracted":false,"has_abstract":true,"ca_institutions":"Xenon Pharmaceuticals (Canada)","funders":"SLAC National Accelerator Laboratory; Basic Energy Sciences; National Institute of General Medical Sciences; Agency for Science, Technology and Research; Office of Science; National Institutes of Health; U.S. Department of Health and Human Services; U.S. Department of Energy","keywords":"T7 RNA polymerase; RNA polymerase; Transcription (linguistics); Polymerase; RNA; Base pair; Chemistry; Computational biology; Bacteriophage; Biology; Biochemistry; DNA; Gene; Escherichia coli","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001149008,0.0002198682,0.0001465151,0.0000659073,0.0001653028,0.0002369999,0.0002606492,0.0003827467,0.000707952],"category_scores_gemma":[0.0002505262,0.0001426028,0.0002015996,0.00004659093,0.0002819577,0.0001942713,0.0001400536,0.0004324789,0.0004189264],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003363125,"about_ca_system_score_gemma":0.0003630726,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000709939,"about_ca_topic_score_gemma":0.0006666245,"domain_scores_codex":[0.9998842,0.00002544512,0.000007365862,0.00002567317,0.00003395393,0.00002331108],"domain_scores_gemma":[0.9998966,0.0000301873,0.00002777603,0.000006659689,0.00001330044,0.00002537112],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008622318,0.00002124888,0.0003391914,0.00005151322,0.000004138444,0.0002373895,0.00003083467,0.0008942263,0.9958417,0.001204367,0.00003247545,0.001256673],"study_design_scores_gemma":[0.00001363949,0.0002471108,0.001219213,0.00000821553,0.000009467667,0.0002902799,0.00006052598,0.0112948,0.9851688,0.0004860235,0.001190585,0.0000112622],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9870481,0.0008669656,0.009185911,0.0001723247,0.00002878402,0.00002844146,0.00006026927,0.00007585662,0.002533346],"genre_scores_gemma":[0.9972199,0.0003238516,0.001959145,0.00003499626,0.000005599049,0.00001240859,0.00007186239,0.000008071508,0.0003641818],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.000709939,"threshold_uncertainty_score":0.002440155,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01519124976838882,"score_gpt":0.2635411771763031,"score_spread":0.2483499274079143,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}