{"id":"W4316190336","doi":"10.1101/2023.01.12.523769","title":"Fluorescent protein lifetimes report increased local densities and phases of nuclear condensates during embryonic stem cell differentiation","year":2023,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Genomics and Chromatin Dynamics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada; HORIZON EUROPE Framework Programme; Israel Science Foundation; Alliance de recherche numérique du Canada; European Commission","keywords":"Fluorescence; Embryonic stem cell; Homogeneous; Fluorescence microscope; Biophysics; Green fluorescent protein; Phase (matter); Induced pluripotent stem cell; Monomer; Chemistry; Biology; Physics; Optics; Polymer; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001636166,0.0002365326,0.0001511347,0.0004348665,0.0001351862,0.0002839927,0.0001708145,0.0002655332,0.001233226],"category_scores_gemma":[0.000226192,0.0001602378,0.0001324826,0.0002692141,0.0003947652,0.0002595772,0.0002581799,0.0005241118,0.0002987165],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003451352,"about_ca_system_score_gemma":0.0001415639,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001318425,"about_ca_topic_score_gemma":0.0009425298,"domain_scores_codex":[0.9998667,0.00001335648,0.00001099477,0.00003334619,0.00005590325,0.00001971749],"domain_scores_gemma":[0.9997663,0.00005789061,0.00006972667,0.0000243258,0.00004579101,0.00003603303],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003095988,0.00000357143,0.0005380505,0.00001168061,0.000002198823,0.0000242126,0.00003188495,0.00005064901,0.9984152,0.00006848166,0.00001834286,0.0008047455],"study_design_scores_gemma":[0.000001973268,0.00001662427,0.005336867,0.000001887129,0.000003571986,0.00004075617,0.00002513191,0.0003389338,0.9937286,0.00004018408,0.0004629517,0.000002469241],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9803097,0.0009584508,0.01615755,0.00007455177,0.00001380981,0.00001420731,0.0006880811,0.00015745,0.001626206],"genre_scores_gemma":[0.9920855,0.0004404828,0.004100337,0.00004105944,0.00000498196,0.0000265899,0.0006019991,0.00007418112,0.002624844],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001318425,"threshold_uncertainty_score":0.004125535,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006576592757049447,"score_gpt":0.1867818724103535,"score_spread":0.180205279653304,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}