{"id":"W4317434112","doi":"10.3390/vaccines11020225","title":"Evaluation of Virus-Free Manufacture of Recombinant Proteins Using CRISPR-Mediated Gene Disruption in Baculovirus-Infected Insect Cells","year":2023,"lang":"en","type":"article","venue":"Vaccines","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Autographa californica; CRISPR; Virology; Biology; Virus; Group-specific antigen; Recombinant DNA; Gene; Baculoviridae; Cell biology; Spodoptera; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009200944,0.0007692119,0.0005952851,0.0004094533,0.0003109127,0.0009520444,0.0005864173,0.0007550012,0.0006325147],"category_scores_gemma":[0.0006006589,0.0002657175,0.0004435304,0.000422145,0.0003193326,0.0003839008,0.0003443461,0.0007860826,0.0005414222],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005120841,"about_ca_system_score_gemma":0.0002860921,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009856828,"about_ca_topic_score_gemma":0.001398738,"domain_scores_codex":[0.9990188,0.0001717116,0.0001564039,0.0001479295,0.0004196714,0.00008552322],"domain_scores_gemma":[0.9996836,0.00007977662,0.00008222372,0.00004844223,0.0000681407,0.00003778146],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003572971,0.00004694951,0.00008712441,0.0000831052,0.000009167987,0.00004867394,0.00002987134,0.0001651085,0.9984855,0.00007622657,0.00003694797,0.0008957208],"study_design_scores_gemma":[0.000007261262,0.0002693615,0.0006769888,0.000006755106,0.0000170131,0.0000729066,0.00001612801,0.0007712973,0.9964364,0.00001819077,0.001701029,0.000006866288],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9500542,0.002322135,0.04111191,0.00014143,0.0001535987,0.0006446433,0.001864137,0.0004689853,0.003238959],"genre_scores_gemma":[0.9304392,0.0026003,0.05630645,0.0001057115,0.00002482121,0.0005373338,0.004753546,0.000193986,0.005038632],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009856828,"threshold_uncertainty_score":0.004865944,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03565403215440578,"score_gpt":0.3073085548403287,"score_spread":0.2716545226859229,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}