{"id":"W4317434112","doi":"10.3390/vaccines11020225","title":"Evaluation of Virus-Free Manufacture of Recombinant Proteins Using CRISPR-Mediated Gene Disruption in Baculovirus-Infected Insect Cells","year":2023,"lang":"en","type":"article","venue":"Vaccines","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Autographa californica; CRISPR; Virology; Biology; Virus; Group-specific antigen; Recombinant DNA; Gene; Baculoviridae; Cell biology; Spodoptera; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005042645,0.0001607394,0.0002102446,0.0002057203,0.00004384407,0.000008578734,0.0001519408,0.0001712388,0.00009458797],"category_scores_gemma":[0.0003371964,0.0001404466,0.000108426,0.0004304724,0.00001968066,0.00001011881,0.0001332327,0.00006717299,0.000006352378],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002911573,"about_ca_system_score_gemma":0.0001246212,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004812551,"about_ca_topic_score_gemma":0.0004478831,"domain_scores_codex":[0.9986196,0.0002255294,0.0003783566,0.0002868022,0.0002967982,0.0001929109],"domain_scores_gemma":[0.9989892,0.0000179748,0.0002271823,0.0004080982,0.0003090589,0.00004845991],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001457322,0.0001529291,0.004903796,0.0000670773,0.00004207937,0.000001005804,0.00005840349,0.004230975,0.9885885,0.000001646066,0.001127748,0.0006801616],"study_design_scores_gemma":[0.001486223,0.000283716,0.04590053,0.00006152059,0.00006551604,0.000002038142,0.00003242519,0.003059269,0.9484215,0.0003195362,0.0002111915,0.0001565398],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9984125,0.0003387607,0.0001239528,0.00002064051,0.0004077275,0.0005388338,0.00008527048,0.00002129018,0.00005101953],"genre_scores_gemma":[0.9990765,0.0003398107,0.0001163008,0.00001900846,0.00008990745,0.00005281368,0.0002485207,0.00002393277,0.00003321975],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.04099673,"threshold_uncertainty_score":0.5727246,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03565403215440578,"score_gpt":0.3073085548403287,"score_spread":0.2716545226859229,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}