{"id":"W4317438730","doi":"10.1021/jacs.2c12666","title":"Engineering a Ligase Binding DNA Aptamer into a Templating DNA Scaffold to Guide the Selective Synthesis of Circular DNAzymes and DNA Aptamers","year":2023,"lang":"en","type":"article","venue":"Journal of the American Chemical Society","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":49,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"Fundamental Research Funds for the Central Universities; Natural Sciences and Engineering Research Council of Canada; Central University Basic Research Fund of China; National Natural Science Foundation of China","keywords":"Aptamer; DNA ligase; Deoxyribozyme; Chemistry; Rolling circle replication; DNA; Sequencing by ligation; In vitro recombination; Biophysics; Combinatorial chemistry; Nanotechnology; Computational biology; Biochemistry; Molecular biology; DNA polymerase; Genomic library; Biology; Molecular cloning; Base sequence; Gene; Complementary DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005397261,0.0001723597,0.0003529923,0.00004463893,0.0001178811,0.00002165439,0.0003146105,0.00007222933,3.466081e-7],"category_scores_gemma":[0.0007730676,0.0001054581,0.0004757031,0.0007093603,0.0002910312,0.000007167176,0.0002420571,0.0001885418,3.765595e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000720762,"about_ca_system_score_gemma":0.00005396735,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000285958,"about_ca_topic_score_gemma":0.000001163523,"domain_scores_codex":[0.9988496,0.00005343798,0.0003816936,0.0002178404,0.0002539674,0.0002435069],"domain_scores_gemma":[0.9988719,0.0001508297,0.0005000912,0.0002473397,0.0001376675,0.00009215879],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002355709,0.00001539759,0.0005331583,0.00001636809,0.000306315,0.000001250868,0.000221824,0.00004189961,0.9939054,0.000001235935,0.002024041,0.002909616],"study_design_scores_gemma":[0.00008070423,0.00007208622,0.0003591796,0.00009199706,0.0001238176,0.0000355938,0.001313839,0.0003036907,0.9962885,0.00001737864,0.0011743,0.0001389243],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9981805,0.00005718169,0.0003846539,0.001226265,0.00002714518,0.00008378644,0.000007605582,0.00001744566,0.0000153881],"genre_scores_gemma":[0.9916531,0.000178322,0.007610265,0.0003520471,0.0001533993,0.000004839027,0.000001502824,0.00002296395,0.00002351459],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.007225611,"threshold_uncertainty_score":0.4300455,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006426886923284086,"score_gpt":0.2556128965188471,"score_spread":0.249186009595563,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}