{"id":"W4317438730","doi":"10.1021/jacs.2c12666","title":"Engineering a Ligase Binding DNA Aptamer into a Templating DNA Scaffold to Guide the Selective Synthesis of Circular DNAzymes and DNA Aptamers","year":2023,"lang":"en","type":"article","venue":"Journal of the American Chemical Society","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":49,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"Fundamental Research Funds for the Central Universities; Natural Sciences and Engineering Research Council of Canada; Central University Basic Research Fund of China; National Natural Science Foundation of China","keywords":"Aptamer; DNA ligase; Deoxyribozyme; Chemistry; Rolling circle replication; DNA; Sequencing by ligation; In vitro recombination; Biophysics; Combinatorial chemistry; Nanotechnology; Computational biology; Biochemistry; Molecular biology; DNA polymerase; Genomic library; Biology; Molecular cloning; Base sequence; Gene; Complementary DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003050905,0.0004812405,0.0003184665,0.0003173318,0.0001875469,0.0003619706,0.0004272613,0.0005651041,0.0008068525],"category_scores_gemma":[0.0004931972,0.0003880456,0.0003752965,0.0002508895,0.0003162361,0.0003578736,0.0003787233,0.0006035512,0.0007866436],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003165893,"about_ca_system_score_gemma":0.000427556,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004032333,"about_ca_topic_score_gemma":0.0006309545,"domain_scores_codex":[0.9997131,0.00003848694,0.00003556098,0.00008854026,0.00006603213,0.00005826393],"domain_scores_gemma":[0.9997094,0.00006140427,0.0001044033,0.00003518911,0.00003499608,0.00005449328],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003114505,0.00003161667,0.000129444,0.00003958043,0.000004599853,0.00008666739,0.00002416651,0.0004469633,0.9962076,0.0003267124,0.00004016291,0.002631337],"study_design_scores_gemma":[0.0000106097,0.0001569812,0.0002239056,0.00000345022,0.000007197029,0.0001858184,0.000009194034,0.002585664,0.9947264,0.00005167693,0.002029077,0.00001009529],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.7885864,0.001071556,0.2040569,0.0003522351,0.0001125968,0.0003305263,0.0003603212,0.001309284,0.003820154],"genre_scores_gemma":[0.8502085,0.0005874524,0.1446364,0.0001756202,0.00002099406,0.0001847416,0.0004635832,0.0001450311,0.003577782],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.0008068525,"threshold_uncertainty_score":0.002699137,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006426886923284086,"score_gpt":0.2556128965188471,"score_spread":0.249186009595563,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}