{"id":"W4317481674","doi":"10.1371/journal.pone.0280353","title":"Small-molecule inhibitors of proteasome increase CjCas9 protein stability","year":2023,"lang":"en","type":"article","venue":"PLoS ONE","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval","funders":"Canadian Institutes of Health Research; Ataxia UK; Ataxia Canada; National Ataxia Foundation","keywords":"Bortezomib; HEK 293 cells; Transfection; MG132; Proteasome; Biology; Molecular biology; Proteasome inhibitor; Transgene; HeLa; Gene; Cell biology; Chemistry; Cell culture; Genetics; Immunology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003245336,0.000522731,0.00052762,0.0002629163,0.0002235577,0.0004220188,0.0001809653,0.0003272356,0.002246881],"category_scores_gemma":[0.0003407118,0.0001467007,0.0003686557,0.0001657073,0.0002465777,0.0002917921,0.0001451325,0.0007857367,0.0003917564],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003571786,"about_ca_system_score_gemma":0.0002520985,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004184026,"about_ca_topic_score_gemma":0.000950342,"domain_scores_codex":[0.9997218,0.00003085608,0.00003868662,0.00007540439,0.00008913927,0.00004405083],"domain_scores_gemma":[0.9998196,0.00004666931,0.00005301146,0.00001878035,0.00002853292,0.00003350387],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000118861,0.00004740258,0.0001246165,0.0001283145,0.00001546395,0.00003254103,0.000007455861,0.00005924514,0.9971097,0.00006665316,0.0001485151,0.002141208],"study_design_scores_gemma":[0.00001815965,0.0001641543,0.001110681,0.000004544585,0.00001579535,0.0001426641,0.000004653417,0.0003360094,0.9953709,0.00002860886,0.002799703,0.000004128676],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9533524,0.0211658,0.01597166,0.0006502497,0.0004550232,0.0001710399,0.001991467,0.0008932318,0.005349133],"genre_scores_gemma":[0.9790654,0.004584742,0.01077287,0.0001865626,0.00005695988,0.00008441873,0.001478681,0.00008292736,0.003687449],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002246881,"threshold_uncertainty_score":0.007516563,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03005647431562459,"score_gpt":0.2487452705044556,"score_spread":0.218688796188831,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}