{"id":"W4317620272","doi":"10.21203/rs.3.pex-1438/v2","title":"cpn60 metagenomic amplicon library preparation for the Illumina Miseq platform","year":2023,"lang":"en","type":"preprint","venue":"Research Square","topic":"Microbial infections and disease research","field":"Immunology and Microbiology","cited_by":2,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Saskatchewan","funders":"","keywords":"Biology; Amplicon; Computational biology; Metagenomics; DNA sequencing; Primer (cosmetics); Genetics; Polymerase chain reaction; Gene; Chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001534993,0.00246892,0.002379544,0.002363971,0.002027018,0.001718203,0.001672803,0.001345578,0.02252285],"category_scores_gemma":[0.003185594,0.001822445,0.002280749,0.002328944,0.0005732089,0.000851261,0.001908051,0.003877407,0.02793154],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008349725,"about_ca_system_score_gemma":0.00250726,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00251143,"about_ca_topic_score_gemma":0.006249638,"domain_scores_codex":[0.9978491,0.0001733916,0.0001544509,0.0008181642,0.0006506873,0.0003542638],"domain_scores_gemma":[0.9991676,0.000162289,0.00005344374,0.0002063034,0.0002704145,0.0001400043],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0007484238,0.0001493109,0.001471375,0.001181781,0.000203515,0.0002248055,0.0002993114,0.001491253,0.9148495,0.003267846,0.03109574,0.04501713],"study_design_scores_gemma":[0.0001483974,0.0005536586,0.008315326,0.0002390239,0.0002531888,0.0004815429,0.0001113791,0.009543756,0.6708014,0.00526273,0.3040735,0.0002161127],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.07318743,0.003142323,0.5750139,0.0009135961,0.002511175,0.005149541,0.2664655,0.03467474,0.0389419],"genre_scores_gemma":[0.06290966,0.001744446,0.5338007,0.001814415,0.0004758257,0.007848225,0.3499829,0.01067444,0.03074952],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.02252285,"threshold_uncertainty_score":0.07534641,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1509251567710007,"score_gpt":0.4200543421708498,"score_spread":0.2691291853998491,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}