{"id":"W4318940233","doi":"10.1016/j.algal.2023.102998","title":"Instability of extrachromosomal DNA transformed into the diatom Phaeodactylum tricornutum","year":2023,"lang":"en","type":"article","venue":"Algal Research","topic":"Microbial Metabolic Engineering and Bioproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":26,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University; Université du Québec à Trois-Rivières","funders":"Natural Sciences and Engineering Research Council of Canada; Mitacs; Canada Research Chairs","keywords":"Phaeodactylum tricornutum; Biology; Green fluorescent protein; mCherry; Extrachromosomal DNA; Expression cassette; Plasmid; Fusion protein; Molecular biology; Biochemistry; DNA; Gene; Recombinant DNA; Vector (molecular biology); Algae","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00019309,0.0003122256,0.0002825462,0.0003863334,0.0001490843,0.0004181064,0.0002409992,0.0003654546,0.0005665162],"category_scores_gemma":[0.0005539247,0.0002051117,0.0002268529,0.0003474238,0.0001746879,0.0002231928,0.000336737,0.0004180568,0.0002351389],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004828168,"about_ca_system_score_gemma":0.0002155886,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002378518,"about_ca_topic_score_gemma":0.002010631,"domain_scores_codex":[0.9997724,0.00003100785,0.00002704176,0.00006162989,0.00008232062,0.00002553204],"domain_scores_gemma":[0.9996061,0.0001111929,0.0001311351,0.00004636238,0.00006182738,0.00004350784],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001400113,0.00000398068,0.0002289658,0.000018269,0.000003009481,0.00002790671,0.00001624677,0.00004350367,0.9989967,0.00002057576,0.000004375017,0.0006224178],"study_design_scores_gemma":[0.000005494115,0.0002305714,0.01122554,0.00001443916,0.00002358911,0.0002610102,0.00007513473,0.001077396,0.9855192,0.00003339554,0.001526188,0.000007932929],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9942059,0.0008796388,0.003356089,0.00005674287,0.00001676926,0.00002624012,0.0004102562,0.00006844026,0.0009799141],"genre_scores_gemma":[0.9918506,0.0006687763,0.002495439,0.00003057237,0.000004397749,0.00004141929,0.001546158,0.0000593007,0.003303336],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002378518,"threshold_uncertainty_score":0.004729331,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03467087567520282,"score_gpt":0.3419413911855755,"score_spread":0.3072705155103727,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}