{"id":"W4318985345","doi":"10.22541/au.167535214.41739207/v1","title":"High-cell-density production of adeno-associated viral vector serotype 6 by triple transfection in suspension HEK293 cell cultures","year":2023,"lang":"en","type":"preprint","venue":"","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Fonds de recherche du Québec – Nature et technologies; Fonds de Recherche du Québec - Santé; Canada Research Chairs; Faculty of Engineering, McGill University","keywords":"Transfection; Genetic enhancement; Cell; Cell culture; Titer; Biology; HEK 293 cells; Transgene; Plasmid; Viral vector; Cell biology; Virology; Virus; Gene; Genetics; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001329145,0.0007761173,0.001106656,0.0006209485,0.0003613657,0.001259701,0.0008555909,0.0009897311,0.001830918],"category_scores_gemma":[0.0006072214,0.0004510444,0.0008419934,0.000566759,0.0004520467,0.0006941612,0.0007549195,0.002551645,0.003260246],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004740596,"about_ca_system_score_gemma":0.0003558713,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005986596,"about_ca_topic_score_gemma":0.0007756053,"domain_scores_codex":[0.9987801,0.0001817406,0.0001785037,0.0002309906,0.0005220058,0.0001067924],"domain_scores_gemma":[0.9996308,0.00007865789,0.00005908585,0.00006793369,0.0001193285,0.0000442441],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002931883,0.00004046176,0.00008500928,0.0001059971,0.00000734892,0.00007453855,0.00005438021,0.0001593167,0.9965035,0.0003661373,0.0002040955,0.002369971],"study_design_scores_gemma":[0.00001261804,0.0001916173,0.0004157159,0.00001889119,0.00002817789,0.0002574989,0.00002548111,0.002339458,0.9869357,0.0001539552,0.009599259,0.00002161045],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.4244319,0.006389226,0.5416508,0.0006282326,0.0008549929,0.001771249,0.005775517,0.003385341,0.0151128],"genre_scores_gemma":[0.5724034,0.006961602,0.3854358,0.000371443,0.0001312828,0.001368323,0.0130135,0.0008534114,0.01946119],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.001830918,"threshold_uncertainty_score":0.007029235,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01561674113762262,"score_gpt":0.2788644564059058,"score_spread":0.2632477152682832,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}