{"id":"W4319296091","doi":"10.34133/bdr.0009","title":"SLICER: A Seamless Gene Deletion Method for Deinococcus radiodurans","year":2023,"lang":"en","type":"article","venue":"BioDesign Research","topic":"Microbial Metabolic Engineering and Bioproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Deinococcus radiodurans; Plasmid; Biology; Synthetic biology; Genome editing; Computational biology; Homing endonuclease; Gene; Homologous recombination; Genome engineering; Genetics; CRISPR; Genome","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005731169,0.0007308432,0.0005448519,0.0005944383,0.0002490446,0.0003712246,0.0005853419,0.0004673298,0.0009761215],"category_scores_gemma":[0.0003548541,0.0004252821,0.0005624696,0.0003452963,0.0002789209,0.0003768054,0.0007297752,0.001048568,0.001292512],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000193572,"about_ca_system_score_gemma":0.0003295194,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000501207,"about_ca_topic_score_gemma":0.0007347045,"domain_scores_codex":[0.999512,0.00007352457,0.00006494414,0.0001338926,0.0001539745,0.00006176598],"domain_scores_gemma":[0.9997119,0.00004814433,0.00009752877,0.00006829402,0.00002970952,0.0000444107],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002639075,0.00001349033,0.00007566678,0.0000415346,0.000004691816,0.00007765103,0.00002902969,0.00004431863,0.9958561,0.0001315729,0.00006458798,0.003634939],"study_design_scores_gemma":[0.000008613085,0.0001157859,0.000733404,0.000009514016,0.00001461381,0.0008147686,0.00001700219,0.000578113,0.9909354,0.00004301031,0.006713558,0.00001623694],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.4338218,0.002775194,0.5528394,0.0004294236,0.0001940479,0.001010273,0.002255247,0.002865914,0.00380869],"genre_scores_gemma":[0.6134259,0.003479409,0.3695306,0.0001593476,0.00003099017,0.0007491544,0.004522648,0.0007409177,0.00736112],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.0009761215,"threshold_uncertainty_score":0.00326544,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.08963727346521379,"score_gpt":0.4119173340954055,"score_spread":0.3222800606301918,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}