{"id":"W4319598353","doi":"10.3390/cells12040545","title":"An Optimized Workflow to Generate and Characterize iPSC-Derived Motor Neuron (MN) Spheroids","year":2023,"lang":"en","type":"article","venue":"Cells","topic":"Pluripotent Stem Cells Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":22,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Montreal Neurological Institute and Hospital","funders":"Canada First Research Excellence Fund","keywords":"Spheroid; Induced pluripotent stem cell; Immunocytochemistry; Neuron; Biology; Workflow; Neuroscience; Profiling (computer programming); Cell; Motor neuron; Progenitor cell; Computer science; Cell biology; Computational biology; Chemistry; In vitro; Stem cell; Gene; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002111437,0.0001807375,0.0001734119,0.00006362854,0.00008922657,0.00008822278,0.0002681471,0.0001295169,0.00006016809],"category_scores_gemma":[0.00002485276,0.000179846,0.00005498105,0.000180086,0.0000510668,0.000006757784,0.0002365192,0.0000939469,0.0002648652],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000009594583,"about_ca_system_score_gemma":0.00003812101,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000006882749,"about_ca_topic_score_gemma":0.00000386383,"domain_scores_codex":[0.998564,0.0001233638,0.0001655975,0.0005414087,0.0001640359,0.0004415848],"domain_scores_gemma":[0.9991149,0.00001305273,0.00003511192,0.0004689735,0.00005595558,0.0003120229],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001831317,0.00003150054,0.00004513127,0.00001705118,0.00001756264,0.00002750694,0.00002914913,0.0002988355,0.9918671,6.962361e-7,0.002500146,0.00498222],"study_design_scores_gemma":[0.0006668484,0.0003803837,0.003868459,0.000008868199,0.000007278275,0.000004168528,0.00002040723,0.002519774,0.9764343,0.000005404174,0.01584783,0.0002362749],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9982527,0.0001001568,0.0004374092,0.0002597214,0.0002429031,0.0003679913,0.00005799763,0.00005078969,0.0002303683],"genre_scores_gemma":[0.981065,0.001077737,0.004795994,0.0006153511,0.00050871,0.00009092354,0.0005169765,0.00009043242,0.01123886],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01718765,"threshold_uncertainty_score":0.7333907,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01759584464023696,"score_gpt":0.2609509441580666,"score_spread":0.2433550995178296,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}