{"id":"W4320482686","doi":"10.1038/s41434-023-00389-y","title":"Sonoselective delivery using ultrasound and microbubbles combined with intravenous rAAV9 CLDN5-GFP does not increase endothelial gene expression","year":2023,"lang":"en","type":"article","venue":"Gene Therapy","topic":"Ultrasound and Hyperthermia Applications","field":"Engineering","cited_by":5,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia; BC Children's Hospital; University of Toronto; Sunnybrook Health Science Centre","funders":"Carlsbergfondet; Government of Canada; National Institute of Biomedical Imaging and Bioengineering; Weston Brain Institute","keywords":"Microbubbles; Gene delivery; Transgene; Genetic enhancement; Biology; Transduction (biophysics); Molecular biology; Gene expression; Blood–brain barrier; Endothelium; Endothelial stem cell; Green fluorescent protein; Cell biology; Pathology; Gene; Medicine; Central nervous system; Ultrasound; Biophysics; In vitro; Neuroscience; Endocrinology; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004428299,0.0004634499,0.000623355,0.000267579,0.000204042,0.0006402858,0.0004083056,0.000460598,0.00228578],"category_scores_gemma":[0.0003223649,0.0003124034,0.000310836,0.0001828181,0.0004865927,0.0005403519,0.0002076147,0.001211969,0.0005611773],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000568256,"about_ca_system_score_gemma":0.0006110668,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003813253,"about_ca_topic_score_gemma":0.004477425,"domain_scores_codex":[0.999512,0.0000869994,0.00003492608,0.0001149345,0.00009923834,0.0001518985],"domain_scores_gemma":[0.9995765,0.0001496165,0.00009165204,0.00005165866,0.00003214734,0.00009839666],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002770193,0.00007990215,0.00008672503,0.00002991394,0.00000954723,0.00003156087,0.00002361755,0.00009481225,0.9974474,0.0002081741,0.00009842615,0.001612952],"study_design_scores_gemma":[0.000015376,0.000253178,0.0004393311,0.000002697647,0.00001636895,0.00005254508,0.00001333394,0.0007442606,0.9974617,0.00001608912,0.0009815344,0.000003498654],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9836962,0.001906201,0.009493131,0.0002836812,0.0001727712,0.00007097144,0.0003190326,0.0003073075,0.003750695],"genre_scores_gemma":[0.9826156,0.001004834,0.007190058,0.0001292312,0.00004268944,0.00005763468,0.0005035145,0.0001508334,0.008305747],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003813253,"threshold_uncertainty_score":0.00764668,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01114958278645808,"score_gpt":0.2063282115550776,"score_spread":0.1951786287686195,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}