{"id":"W4320482686","doi":"10.1038/s41434-023-00389-y","title":"Sonoselective delivery using ultrasound and microbubbles combined with intravenous rAAV9 CLDN5-GFP does not increase endothelial gene expression","year":2023,"lang":"en","type":"article","venue":"Gene Therapy","topic":"Ultrasound and Hyperthermia Applications","field":"Engineering","cited_by":5,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia; BC Children's Hospital; University of Toronto; Sunnybrook Health Science Centre","funders":"Carlsbergfondet; Government of Canada; National Institute of Biomedical Imaging and Bioengineering; Weston Brain Institute","keywords":"Microbubbles; Gene delivery; Transgene; Genetic enhancement; Biology; Transduction (biophysics); Molecular biology; Gene expression; Blood–brain barrier; Endothelium; Endothelial stem cell; Green fluorescent protein; Cell biology; Pathology; Gene; Medicine; Central nervous system; Ultrasound; Biophysics; In vitro; Neuroscience; Endocrinology; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0000965078,0.000237267,0.0002262323,0.0001108597,0.0002464269,0.0000727591,0.0001299377,0.00008790451,0.00003256925],"category_scores_gemma":[0.000005624728,0.0001749065,0.00003964436,0.0002374701,0.00007606966,0.0001011805,0.00001714782,0.0001287319,0.00001677142],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005091456,"about_ca_system_score_gemma":0.00004084358,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001159557,"about_ca_topic_score_gemma":0.0000441154,"domain_scores_codex":[0.9990712,0.00003928474,0.0001657642,0.0002725392,0.0001228761,0.0003283431],"domain_scores_gemma":[0.9994154,0.0001630535,0.00003735015,0.0002370068,0.00004765821,0.00009951357],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002372721,0.00004295744,0.0008004554,0.00001162008,0.000096785,0.000006472167,0.001058246,0.0026347,0.9924797,0.00001421875,0.0000468608,0.002570646],"study_design_scores_gemma":[0.001387551,0.0001219366,0.003319069,0.00002039914,0.00002481191,0.0000577415,0.0001595901,0.001095626,0.9926296,0.0002347817,0.0006437433,0.0003051826],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9968346,0.00047867,0.001622078,0.00002158441,0.00007957024,0.0003600122,0.0001411952,0.0004140819,0.00004816483],"genre_scores_gemma":[0.9918166,0.00318886,0.004416744,0.00009662435,0.0001995601,0.00006650962,0.00006860614,0.00008014968,0.00006633288],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00501803,"threshold_uncertainty_score":0.7132481,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01114958278645808,"score_gpt":0.2063282115550776,"score_spread":0.1951786287686195,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}