{"id":"W4321763815","doi":"10.3791/64793","title":"Rapid Development of Cell State Identification Circuits with Poly-Transfection","year":2023,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"British Columbia Institute of Technology; University of British Columbia","funders":"National Institute of General Medical Sciences; National Cancer Institute","keywords":"Transfection; Flow cytometry; Electronic circuit; Population; Computer science; Cell culture; Biological system; Biology; Molecular biology; Physics; Genetics; Medicine","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000648929,0.0007309801,0.0006081663,0.0004354742,0.0001903015,0.0008779516,0.001337897,0.0006402099,0.002543421],"category_scores_gemma":[0.001016183,0.000472973,0.0005248131,0.0003349191,0.0004580991,0.0006904701,0.0005969495,0.001428611,0.001621786],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006035178,"about_ca_system_score_gemma":0.0003227108,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002473642,"about_ca_topic_score_gemma":0.0005068463,"domain_scores_codex":[0.9992394,0.00008001594,0.00008166527,0.0001934438,0.0003432421,0.00006210693],"domain_scores_gemma":[0.9994523,0.0001476721,0.0001207116,0.00009173605,0.0001445555,0.0000431218],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009259202,0.00008853598,0.0001832714,0.0001531462,0.00002092481,0.00009361086,0.00003989199,0.002144071,0.9610253,0.004145573,0.0008461075,0.03116701],"study_design_scores_gemma":[0.00002822262,0.0003304964,0.0004051023,0.0000193091,0.00003230491,0.0001569625,0.000008505397,0.0205208,0.9585782,0.0006342198,0.01926127,0.00002453853],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.05903974,0.001100773,0.9236603,0.0003860203,0.0005036896,0.0007056107,0.0004737185,0.00618655,0.00794368],"genre_scores_gemma":[0.3658183,0.001437264,0.615163,0.0008186148,0.00009275095,0.002155026,0.001166796,0.0005296841,0.01281851],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002543421,"threshold_uncertainty_score":0.008508563,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01788568460380941,"score_gpt":0.3852519049928391,"score_spread":0.3673662203890297,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}