{"id":"W4323810846","doi":"10.1126/science.abq4822","title":"Droplet-based forward genetic screening of astrocyte–microglia cross-talk","year":2023,"lang":"en","type":"article","venue":"Science","topic":"Single-cell and spatial transcriptomics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":117,"is_retracted":false,"has_abstract":true,"ca_institutions":"Centre Hospitalier de l’Université de Montréal; McGill University; Montreal Neurological Institute and Hospital","funders":"National Institute of Environmental Health Sciences; National Institute of Arthritis and Musculoskeletal and Skin Diseases; National Institute of Allergy and Infectious Diseases; National Institute of Mental Health; National Institute of Neurological Disorders and Stroke; National Institute of General Medical Sciences; Helmholtz Zentrum München; National Institutes of Health; Eli Lilly and Company; European Foundation for the Study of Diabetes; International Progressive MS Alliance; Fonds de Recherche du Québec - Santé; Crohn's and Colitis Foundation; National Research Foundation of Korea; National Cancer Institute; National Research Foundation; Brigham and Women's Hospital; Deutsche Forschungsgemeinschaft; National Multiple Sclerosis Society","keywords":"Microglia; CRISPR; Astrocyte; Cell; Computational biology; Biology; Cell type; Genetic screen; Cell sorting; Neuroscience; Identification (biology); High-throughput screening; Central nervous system; Bioinformatics; Genetics; Immunology; Phenotype; Gene; Inflammation","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004445863,0.0004280707,0.000443502,0.0002099708,0.0002178878,0.0003170916,0.0003778545,0.0004139904,0.0008012979],"category_scores_gemma":[0.000284436,0.0001943213,0.0003249221,0.000152797,0.0003147409,0.000239682,0.0005313217,0.0005609802,0.0002957258],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000402326,"about_ca_system_score_gemma":0.0002912256,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004123191,"about_ca_topic_score_gemma":0.000869353,"domain_scores_codex":[0.9996018,0.0000414034,0.00002166033,0.0001081972,0.0001818982,0.00004511026],"domain_scores_gemma":[0.9998037,0.00007526758,0.00004385156,0.00002334404,0.00003302043,0.00002079175],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00000911884,0.000004622345,0.00003952584,0.0000118885,0.000002232983,0.00001110444,0.000003951865,0.0000937111,0.9992521,0.00006048603,0.00001331449,0.0004977192],"study_design_scores_gemma":[0.000002958217,0.00003960147,0.0003490554,0.000001282165,0.000004631057,0.00003306735,0.000005741659,0.00224261,0.9968398,0.00005222919,0.0004255219,0.00000348748],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8586491,0.001069209,0.1344918,0.0001903082,0.00009372368,0.0003357177,0.001872096,0.0009411963,0.002356808],"genre_scores_gemma":[0.9074451,0.001119835,0.08711313,0.0001495369,0.00001430104,0.0003290437,0.001112594,0.00016059,0.002555815],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008012979,"threshold_uncertainty_score":0.002919137,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01585219179612322,"score_gpt":0.2756237908501996,"score_spread":0.2597715990540763,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}