{"id":"W4323810846","doi":"10.1126/science.abq4822","title":"Droplet-based forward genetic screening of astrocyte–microglia cross-talk","year":2023,"lang":"en","type":"article","venue":"Science","topic":"Single-cell and spatial transcriptomics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":117,"is_retracted":false,"has_abstract":true,"ca_institutions":"Centre Hospitalier de l’Université de Montréal; McGill University; Montreal Neurological Institute and Hospital","funders":"National Institute of Environmental Health Sciences; National Institute of Arthritis and Musculoskeletal and Skin Diseases; National Institute of Allergy and Infectious Diseases; National Institute of Mental Health; National Institute of Neurological Disorders and Stroke; National Institute of General Medical Sciences; Helmholtz Zentrum München; National Institutes of Health; Eli Lilly and Company; European Foundation for the Study of Diabetes; International Progressive MS Alliance; Fonds de Recherche du Québec - Santé; Crohn's and Colitis Foundation; National Research Foundation of Korea; National Cancer Institute; National Research Foundation; Brigham and Women's Hospital; Deutsche Forschungsgemeinschaft; National Multiple Sclerosis Society","keywords":"Microglia; CRISPR; Astrocyte; Cell; Computational biology; Biology; Cell type; Genetic screen; Cell sorting; Neuroscience; Identification (biology); High-throughput screening; Central nervous system; Bioinformatics; Genetics; Immunology; Phenotype; Gene; Inflammation","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002861634,0.00009767863,0.00009765833,0.00008883877,0.0001527966,0.00004503131,0.0003982137,0.000058485,0.00001318486],"category_scores_gemma":[0.00007986639,0.00009455882,0.00007540148,0.0004744403,0.0005784957,0.000005538426,0.00007726862,0.00005100365,0.00001322011],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00000828774,"about_ca_system_score_gemma":0.0001709428,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000313433,"about_ca_topic_score_gemma":0.0000160628,"domain_scores_codex":[0.998904,0.00001298397,0.0001713169,0.0003434953,0.0002396438,0.0003285356],"domain_scores_gemma":[0.9994354,0.000008324992,0.00005778962,0.0002949153,0.0001169799,0.00008663991],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002782312,0.00001465987,0.02222155,0.0000126463,0.000003550131,0.000002605442,0.00001718387,0.001516155,0.9726277,0.000006146074,0.00005233935,0.003497632],"study_design_scores_gemma":[0.0003480453,0.0001680785,0.05229375,0.00001264067,0.00000467372,0.000003171318,0.00002155817,0.003721862,0.94015,0.00001755028,0.003141612,0.000117048],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9835724,0.0001278292,0.0157381,0.00003317895,0.0002014049,0.00007874435,0.00001892588,0.00002270526,0.0002067006],"genre_scores_gemma":[0.9932821,0.00001214626,0.006197886,0.0001002469,0.00006463019,0.000004860377,0.00001882723,0.00001188828,0.0003073733],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0324777,"threshold_uncertainty_score":0.3855997,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01585219179612322,"score_gpt":0.2756237908501996,"score_spread":0.2597715990540763,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}