{"id":"W4324026231","doi":"10.1016/j.xpro.2023.102152","title":"Protocol for in vitro phosphorylation of the MUS81-binding region of SLX4 using CDK1-cyclin B","year":2023,"lang":"en","type":"article","venue":"STAR Protocols","topic":"Digestive system and related health","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Canada Research Chairs; University of Toronto","funders":"Scheme for Promotion of Academic and Research Collaboration; Canadian Institutes of Health Research; Hospital for Sick Children; Canada Research Chairs","keywords":"Cyclin-dependent kinase 1; Protocol (science); Cell biology; Cyclin B; In vitro; Chemistry; Cyclin; Biology; Cell cycle; Medicine; Biochemistry; Cell","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006667731,0.001752058,0.0009233957,0.001047288,0.001232659,0.0004790227,0.001444618,0.0004340622,0.02127912],"category_scores_gemma":[0.0004215625,0.001033869,0.0006509353,0.001042397,0.0003607769,0.0005067407,0.000672431,0.002563469,0.01575868],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007378641,"about_ca_system_score_gemma":0.001167326,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001404331,"about_ca_topic_score_gemma":0.00470663,"domain_scores_codex":[0.9993482,0.0001029448,0.00007964687,0.0001564173,0.0002231626,0.00008960294],"domain_scores_gemma":[0.9996902,0.00006372695,0.00002722171,0.00009706928,0.00007266584,0.00004917154],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0002852732,0.0001757824,0.0002130306,0.0003691202,0.00003011831,0.0001475779,0.0000793869,0.0003906491,0.9798116,0.001736761,0.009175993,0.007584759],"study_design_scores_gemma":[0.00018213,0.000674856,0.004174668,0.00007772857,0.00008721291,0.0007585999,0.00004707921,0.003252162,0.7400042,0.001336087,0.2493167,0.00008855143],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"protocol","genre_scores_codex":[0.1200735,0.00686863,0.7680648,0.001404394,0.001443522,0.01005752,0.04219398,0.009137205,0.04075646],"genre_scores_gemma":[0.1307472,0.01113554,0.5167347,0.001014641,0.0002879977,0.02373828,0.1964998,0.002323679,0.1175181],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.02127912,"threshold_uncertainty_score":0.07118577,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.07207337346745758,"score_gpt":0.387475432909711,"score_spread":0.3154020594422535,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}