{"id":"W4324342851","doi":"10.1016/j.omtm.2023.03.006","title":"Simultaneous engineering of natural killer cells for CAR transgenesis and CRISPR-Cas9 knockout using retroviral particles","year":2023,"lang":"en","type":"article","venue":"Molecular Therapy — Methods & Clinical Development","topic":"CAR-T cell therapy research","field":"Medicine","cited_by":35,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada; Ottawa Hospital; Institute of Infection and Immunity; University of Ottawa","funders":"National Cancer Institute; National Research Council Canada; Ottawa Hospital Research Institute; Canadian Institutes of Health Research; University of Ottawa","keywords":"CRISPR; Transgenesis; Biology; Cas9; Genetics; Computational biology; Gene; Reproductive technology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.003293771,0.000304907,0.0008299676,0.0002152318,0.00008624936,0.00002664267,0.0001718834,0.000225027,0.00007857849],"category_scores_gemma":[0.0008073324,0.0002645172,0.0003568471,0.000490892,0.0001324296,0.00003442465,0.00007400356,0.0003394766,0.000005185362],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00008787936,"about_ca_system_score_gemma":0.000234713,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001256472,"about_ca_topic_score_gemma":0.000001218296,"domain_scores_codex":[0.9968852,0.0004619888,0.001011886,0.000577914,0.0004655818,0.0005973759],"domain_scores_gemma":[0.9967629,0.002227393,0.0001202776,0.0003891642,0.000225386,0.0002748271],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0007429131,0.00008312243,0.000385196,0.0001064208,0.0003924666,0.00006080596,0.0004504201,0.0004069698,0.6632996,0.000008974609,0.00001451356,0.3340486],"study_design_scores_gemma":[0.003597673,0.0005373242,0.003151087,0.00009899824,0.00009266668,0.00001536977,0.000103555,0.0386411,0.9276743,0.00006989024,0.02566931,0.0003487403],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.8966511,0.001462965,0.09994093,0.0002048624,0.0005979339,0.001032664,0.000003297232,0.00009709768,0.000009122493],"genre_scores_gemma":[0.4564626,0.0007701081,0.5414327,0.0003068174,0.00008949675,0.00006399801,0.00001447544,0.0001034118,0.0007564027],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.4414918,"threshold_uncertainty_score":0.9999807,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1189124007392817,"score_gpt":0.4657807527445325,"score_spread":0.3468683520052508,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}