{"id":"W4327575945","doi":"10.12688/f1000research.130991.1","title":"The identification of high-performing antibodies for Secreted frizzled-related protein 1 (sFRP-1) for use in Western Blot and immunoprecipitation","year":2023,"lang":"en","type":"preprint","venue":"F1000Research","topic":"Cancer-related gene regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Montreal Neurological Institute and Hospital","funders":"Genentech; Innovative Medicines Initiative; Government of Canada; Ontario Genomics Institute; Emory University; European Federation of Pharmaceutical Industries and Associations; Merck KGaA; Mitacs; Ontario Genomics; Genome Canada; Bristol-Myers Squibb; Bayer; Pfizer","keywords":"Immunoprecipitation; Western blot; Antibody; Biology; Wnt signaling pathway; Frizzled; Blot; Immunology; Molecular biology; Cell biology; Gene; Signal transduction; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001583016,0.0001840164,0.0002283199,0.000211284,0.0001786604,0.0001267519,0.000281941,0.0004514728,0.00000111902],"category_scores_gemma":[0.0008629418,0.0001686948,0.00009572633,0.00016199,0.0001652621,0.00001299574,0.0003880879,0.0002452404,0.000001573519],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006776177,"about_ca_system_score_gemma":0.0002029558,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000370473,"about_ca_topic_score_gemma":0.0008884201,"domain_scores_codex":[0.9980874,0.0001250343,0.0006839618,0.0005318627,0.0002564185,0.0003153434],"domain_scores_gemma":[0.9983186,0.0002414685,0.0003369203,0.0005290275,0.0005388416,0.00003517901],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0008347453,0.00003023645,0.002552912,0.0005920396,0.0002115019,2.366135e-7,0.0002778704,0.003746037,0.9836321,0.0002142289,0.0002736063,0.007634507],"study_design_scores_gemma":[0.002222052,0.0004767744,0.09881622,0.0005633335,0.00007314439,0.000001989669,0.0002106321,0.0379164,0.8510885,0.006779852,0.001421593,0.0004294869],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9879534,0.00116178,0.005712466,0.000366386,0.0002908418,0.004197522,0.0002934505,0.00002187236,0.000002283718],"genre_scores_gemma":[0.9910709,0.0009020203,0.0008370943,0.000002132035,0.0001146342,0.001663908,0.002553218,0.00007433737,0.002781736],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1325435,"threshold_uncertainty_score":0.6879172,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03931388660979182,"score_gpt":0.3358131913428206,"score_spread":0.2964993047330288,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}