{"id":"W4361269692","doi":"10.1021/acs.jproteome.2c00790","title":"CRISPRi-Mediated Silencing of <i>Burkholderia</i> O-Linked Glycosylation Systems Enables the Depletion of Glycosylation Yet Results in Modest Proteome Impacts","year":2023,"lang":"en","type":"article","venue":"Journal of Proteome Research","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Health and Medical Research Council; Institute of Infection and Immunity; Australian Research Council","keywords":"Glycosylation; Burkholderia; Biology; Proteome; N-linked glycosylation; Burkholderia cenocepacia; Burkholderia cepacia complex; Gene silencing; Cell biology; Glycan; Microbiology; Biochemistry; Genetics; Glycoprotein; Gene; Bacteria","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0054271,0.0001227529,0.0002698706,0.0005079459,0.00006430536,0.00003194873,0.000288143,0.0001770568,0.000003064853],"category_scores_gemma":[0.002837542,0.00009157554,0.00009119699,0.0009246753,0.00006576691,0.00002190168,0.000104153,0.0003695655,0.000003253284],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006538316,"about_ca_system_score_gemma":0.0002712662,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002866857,"about_ca_topic_score_gemma":0.00004313904,"domain_scores_codex":[0.9974087,0.0003564621,0.0009179735,0.0001859816,0.0007790019,0.000351892],"domain_scores_gemma":[0.9981591,0.0002142188,0.0004344424,0.0003027287,0.0008064929,0.00008298631],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005848735,0.00004317609,0.0009240675,0.0002921397,0.00003129909,0.000006751897,0.0003253344,0.03682527,0.9605674,0.000007984951,0.0002023633,0.0001893536],"study_design_scores_gemma":[0.001541279,0.001017602,0.02391066,0.0006221063,0.00001033634,0.00002820643,0.0005933589,0.02063912,0.9510879,0.000105554,0.0003154573,0.0001284022],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9945548,0.0007725303,0.003240581,0.0002613744,0.0001230157,0.0009543301,0.00003499354,0.000006014463,0.00005241989],"genre_scores_gemma":[0.9986501,0.0006934779,0.0003128261,0.000002263593,0.0001921974,0.00004045479,0.00005091691,0.00002264862,0.00003513359],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02298659,"threshold_uncertainty_score":0.3734342,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04979084381550405,"score_gpt":0.3831468336019912,"score_spread":0.3333559897864872,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}