{"id":"W4361269692","doi":"10.1021/acs.jproteome.2c00790","title":"CRISPRi-Mediated Silencing of <i>Burkholderia</i> O-Linked Glycosylation Systems Enables the Depletion of Glycosylation Yet Results in Modest Proteome Impacts","year":2023,"lang":"en","type":"article","venue":"Journal of Proteome Research","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Health and Medical Research Council; Institute of Infection and Immunity; Australian Research Council","keywords":"Glycosylation; Burkholderia; Biology; Proteome; N-linked glycosylation; Burkholderia cenocepacia; Burkholderia cepacia complex; Gene silencing; Cell biology; Glycan; Microbiology; Biochemistry; Genetics; Glycoprotein; Gene; Bacteria","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001383876,0.0003369074,0.0001879782,0.000131897,0.000149236,0.0004194683,0.0002139351,0.0002596534,0.0008178673],"category_scores_gemma":[0.0001593261,0.0001275049,0.000187328,0.0001480472,0.0002733644,0.0001813539,0.0003380937,0.00049144,0.0003568153],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002938005,"about_ca_system_score_gemma":0.000285766,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008849326,"about_ca_topic_score_gemma":0.001278383,"domain_scores_codex":[0.9998399,0.00002047272,0.00001726648,0.0000454014,0.00004581578,0.0000311095],"domain_scores_gemma":[0.9998878,0.00001815823,0.00004401602,0.00001853217,0.00001065109,0.00002087367],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001238836,0.000005118177,0.0001499586,0.00001308833,0.000001944335,0.00002342063,0.000006846142,0.0000501931,0.9990225,0.00008019189,0.00001530181,0.000619146],"study_design_scores_gemma":[0.000002263373,0.0000333923,0.001859537,0.000002883216,0.000004592753,0.0001064777,0.00001817088,0.0006792606,0.9952307,0.00005449473,0.002004539,0.000003587581],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9752331,0.0005444568,0.01895077,0.0001488642,0.00003224939,0.00005242261,0.00100731,0.0004348715,0.0035961],"genre_scores_gemma":[0.9898075,0.0003338592,0.006569132,0.00005720251,0.000003151312,0.00002768743,0.0007312745,0.00004365708,0.002426434],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008849326,"threshold_uncertainty_score":0.002736032,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04979084381550405,"score_gpt":0.3831468336019912,"score_spread":0.3333559897864872,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}