{"id":"W4361800518","doi":"10.2139/ssrn.4397238","title":"Development of an Inducible Cas9 Nickase and Pam-Free Cas12a Platform for Bacterial Diagnostics","year":2023,"lang":"en","type":"article","venue":"SSRN Electronic Journal","topic":"Biosensors and Analytical Detection","field":"Engineering","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"Government of Canada; Agriculture and Agri-Food Canada","funders":"","keywords":"CRISPR; Cas9; Computational biology; Genome editing; Biology; Genetics; Computer science; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004355616,0.0006230504,0.000617902,0.0003567475,0.0002762867,0.000849185,0.0009714456,0.001021961,0.00184934],"category_scores_gemma":[0.00037176,0.0004090514,0.0004755052,0.0002515616,0.0002240809,0.0005599663,0.000553645,0.001280353,0.001927312],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005220792,"about_ca_system_score_gemma":0.0005018438,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006022445,"about_ca_topic_score_gemma":0.001047225,"domain_scores_codex":[0.9994575,0.00004055439,0.00003516874,0.0001284818,0.0002858597,0.00005243396],"domain_scores_gemma":[0.9998106,0.00002362799,0.00004561224,0.00002704362,0.0000488813,0.00004418568],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002648855,0.00001909331,0.00005145872,0.00005095514,0.000006537776,0.00003901948,0.00001153519,0.00008296296,0.9938635,0.0002573135,0.0002047801,0.00538636],"study_design_scores_gemma":[0.000004531469,0.00007720491,0.0001707352,0.000003499328,0.000008088825,0.0001847322,0.000006756617,0.001511581,0.9935029,0.00005179265,0.004467626,0.0000105853],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5113531,0.005113477,0.4584093,0.001212674,0.0006550751,0.0009934572,0.002726916,0.007344975,0.01219107],"genre_scores_gemma":[0.6410545,0.002281181,0.3288233,0.0003418293,0.0000569496,0.0003728794,0.003142783,0.0003142542,0.02361227],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00184934,"threshold_uncertainty_score":0.006186724,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01513603869843186,"score_gpt":0.2288565882175782,"score_spread":0.2137205495191464,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}