{"id":"W4362614894","doi":"10.3390/jdb11020017","title":"Selecting Normalizers for MicroRNA RT-qPCR Expression Analysis in Murine Preimplantation Embryos and the Associated Conditioned Culture Media","year":2023,"lang":"en","type":"article","venue":"Journal of Developmental Biology","topic":"MicroRNA in disease regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"Children’s Health Research Institute; Lawson Health Research Institute; Western University","funders":"Lawson Health Research Institute; Natural Sciences and Engineering Research Council of Canada; Children's Health Research Institute","keywords":"Biology; Blastocyst; Embryo; microRNA; Normalization (sociology); Computational biology; Real-time polymerase chain reaction; Andrology; Cell biology; Genetics; Embryogenesis; Gene; Medicine","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.01421629,0.003031743,0.002522253,0.003800939,0.002084147,0.002611567,0.002016842,0.001515791,0.0072472],"category_scores_gemma":[0.01590015,0.00210137,0.00195688,0.004066996,0.002683862,0.001450927,0.001905082,0.003571128,0.004508964],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001548689,"about_ca_system_score_gemma":0.00267478,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001447633,"about_ca_topic_score_gemma":0.004443266,"domain_scores_codex":[0.9801695,0.004191113,0.002540835,0.005764768,0.00638719,0.0009466389],"domain_scores_gemma":[0.9934534,0.002094595,0.0007324472,0.001209673,0.002383617,0.0001262164],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005451053,0.0002801698,0.003149113,0.0009748484,0.0001137667,0.0001291619,0.0009525608,0.001272861,0.9662154,0.002379627,0.00134392,0.02264342],"study_design_scores_gemma":[0.00004965464,0.0007580279,0.007697764,0.0002215334,0.0002696579,0.0002893068,0.0002961311,0.007579968,0.9553165,0.001423702,0.02598915,0.0001086722],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1249844,0.003989806,0.8424512,0.0003594487,0.001404437,0.005189112,0.004818738,0.00725836,0.009544482],"genre_scores_gemma":[0.07322126,0.002690656,0.8854541,0.0005672805,0.0001416181,0.01812785,0.007997877,0.003755852,0.00804348],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01421629,"threshold_uncertainty_score":0.07518381,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009561623371124388,"score_gpt":0.2650508351196542,"score_spread":0.2554892117485298,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}