{"id":"W4362728512","doi":"10.17504/protocols.io.rm7vzbm3xvx1/v1","title":"Single strain culture DNA extractions with polyzyme v1","year":2022,"lang":"en","type":"preprint","venue":"","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"","keywords":"Strain (injury); DNA; Chemistry; Genetics; Biology; Anatomy","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001022126,0.002468969,0.001862651,0.002377854,0.00114399,0.0009145252,0.002190836,0.001098821,0.01827897],"category_scores_gemma":[0.001603897,0.001751087,0.001252583,0.002363847,0.0006858537,0.0005554029,0.0008529706,0.004239571,0.03009755],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005250986,"about_ca_system_score_gemma":0.001122562,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001452304,"about_ca_topic_score_gemma":0.003155089,"domain_scores_codex":[0.9984729,0.0002157732,0.0001848533,0.0004946976,0.0004221762,0.0002095574],"domain_scores_gemma":[0.9989052,0.0002341072,0.00008546671,0.0004607122,0.0002092929,0.0001051658],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001668975,0.0001337375,0.0002703044,0.0007075383,0.00004842544,0.0001406369,0.0001355391,0.0002379768,0.9730905,0.001102582,0.007392546,0.0165734],"study_design_scores_gemma":[0.0000713466,0.000226935,0.001603595,0.0001133408,0.0001026639,0.0004529453,0.00003911119,0.000987638,0.9009051,0.0009316473,0.09449945,0.00006623347],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.05011825,0.002784352,0.8541893,0.0006184658,0.0008091439,0.007403242,0.05413904,0.01325859,0.01667958],"genre_scores_gemma":[0.06741295,0.004713807,0.6707167,0.000690977,0.0002604413,0.01427657,0.1744864,0.004527315,0.0629148],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01827897,"threshold_uncertainty_score":0.06114918,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01426716838261511,"score_gpt":0.2543212148121727,"score_spread":0.2400540464295576,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}