{"id":"W4365398991","doi":"10.17504/protocols.io.36wgq72n3vk5/v2","title":"HTTM : Transposon mutagenesis v2","year":2022,"lang":"en","type":"preprint","venue":"","topic":"Bacteriophages and microbial interactions","field":"Environmental Science","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Sherbrooke","funders":"","keywords":"Transposon mutagenesis; Transposable element; Mutagenesis; Sleeping Beauty transposon system; Insertional mutagenesis; DNA Transposable Elements; Genetics; Site-directed mutagenesis; Biology; Mutation; Computational biology; Mutant; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001121728,0.002281124,0.001657661,0.002352785,0.00086647,0.001025245,0.001957263,0.001313384,0.05016965],"category_scores_gemma":[0.001351417,0.001505093,0.001067466,0.001832372,0.0006399946,0.0008095083,0.001513015,0.004131354,0.06292909],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004700201,"about_ca_system_score_gemma":0.0007376222,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001366003,"about_ca_topic_score_gemma":0.001831082,"domain_scores_codex":[0.998659,0.0002822107,0.0001235507,0.0002864846,0.0004786454,0.0001700478],"domain_scores_gemma":[0.9993762,0.0001596534,0.00004502604,0.0002736015,0.00007045048,0.0000750929],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0007936801,0.0002494757,0.0004359589,0.001516149,0.0001445324,0.0004780498,0.0003039182,0.0009249877,0.7680338,0.01135268,0.1391654,0.07660121],"study_design_scores_gemma":[0.0002983979,0.0003048273,0.001967868,0.0001161388,0.0001071865,0.001723321,0.00005030338,0.003083545,0.6127656,0.005899521,0.3735244,0.0001589975],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"protocol","genre_scores_codex":[0.02050369,0.003067177,0.7030621,0.000792646,0.001527829,0.002708334,0.1645053,0.07258295,0.03124997],"genre_scores_gemma":[0.09012973,0.004406211,0.4327266,0.000719886,0.0003052159,0.005133849,0.3170661,0.04057191,0.1089404],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.05016965,"threshold_uncertainty_score":0.1678342,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01261436221666311,"score_gpt":0.2449864848620209,"score_spread":0.2323721226453578,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}