{"id":"W4365509357","doi":"10.12688/f1000research.133479.1","title":"The identification of high-performing antibodies for Coiled-coil-helix-coiled-coil-helix domain containing protein 10 (CHCHD10) for use in Western Blot, immunoprecipitation and immunofluorescence","year":2023,"lang":"en","type":"preprint","venue":"F1000Research","topic":"Amyotrophic Lateral Sclerosis Research","field":"Medicine","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Montreal Neurological Institute and Hospital","funders":"Canadian Institutes of Health Research; Mitacs; Ontario Genomics; Genome Canada","keywords":"Immunoprecipitation; Western blot; Antibody; Immunofluorescence; Coiled coil; Blot; Biology; Cell biology; Molecular biology; Gene; Immunology; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001825118,0.002091068,0.0006640169,0.001706878,0.001036777,0.001008079,0.001124201,0.001171554,0.01903802],"category_scores_gemma":[0.00255705,0.0008127,0.0007107577,0.001051041,0.0005727935,0.0007547466,0.000807202,0.001612107,0.01788134],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001086125,"about_ca_system_score_gemma":0.0008208096,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001375226,"about_ca_topic_score_gemma":0.004367492,"domain_scores_codex":[0.99882,0.0002338451,0.0001814918,0.0002389789,0.0003667647,0.0001589164],"domain_scores_gemma":[0.9985381,0.0003723243,0.0001417142,0.000285833,0.0005281701,0.0001337844],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001657359,0.00004660635,0.0006180113,0.0003571036,0.00002343195,0.000173946,0.00007996491,0.0001295428,0.9741791,0.0009627445,0.00916121,0.0141025],"study_design_scores_gemma":[0.00006630079,0.0001487445,0.008176217,0.0001157174,0.00004970892,0.00149023,0.00008936682,0.00158598,0.8722559,0.0006646948,0.1153136,0.00004353763],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.3583831,0.01577661,0.4898752,0.008267357,0.004307354,0.002308714,0.03087673,0.01174195,0.078463],"genre_scores_gemma":[0.2265488,0.007783097,0.6411862,0.001875898,0.0004401262,0.002705892,0.05460684,0.003757611,0.06109546],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.01903802,"threshold_uncertainty_score":0.06368852,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.08249040749477722,"score_gpt":0.3702383076021669,"score_spread":0.2877479001073897,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}